2025

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    4. Yudian Xiao, Bowen Wu, Qiuping Zhou, Fan Wu, Nei Li, Chenqi Xu, Xuexiang Han*. Technologies for Chimeric Antigen Receptor Transgene Delivery. Trends in Molecular Medicine, 2025, DOI: 10.1016/j.molmed.2025.05.011. [Link]

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    3. Fan Wu, Nei Li, Yudian Xiao, Rohan Palanki, Hannah Yamagata, Michael J. Mitchell*, Xuexiang Han*. Lipid Nanoparticles for Delivery of CRISPR Gene Editing Components. Small Methods, 2025, 2401632. [Link]

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    2. Sijia Xu+, Zhenzhen Hu+, Fenglin Song+, Ying Xu*, Xuexiang Han*. Lipid Nanoparticles: Composition, Formulation and Application. Molecular Therapy - Methods & Clinical Development, 2025, 33, 101463. [Link]

    TBD


    1. Xuexiang Han+, Ying Xu+, Adele Ricciardi+, Junchao Xu+, Rohan Palanki, Vivek Chowdhary, Lulu Xue, Ningqiang Gong, Mohamad-Gabriel Alameh, William H. Peranteau, James M. Wilson, Drew Weissman, Michael J. Mitchell*. Plug-and-play assembly of biodegradable ionizable lipids for potent mRNA delivery and gene editing in vivo. bioRxiv, 2025: 2025.02.25.640222.

2024

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    		4. Xuexiang Han, Mohamad-Gabriel Alameh, Ying Xu, Rohan Palanki, Rakan El-Mayta, Garima Dwivedi, Kelsey L. Swingle, Junchao Xu, Ningqiang Gong, Lulu Xue, Qiangqiang Shi, Il-Chul Yoon, Claude C. Warzecha, James M. Wilson, Drew Weissman, Michael J. Mitchell*. Optimization of the activity and biodegradability of ionizable lipids for mRNA delivery via directed chemical evolution. Nature Biomedical Engineering, 2024, 8, 1412–1424. [Link]

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    		3. Yijia Hao, Xuexiang Han*. Research  progress of mRNA-LNP cancer vaccines. Chemistry of Life, 2024, 44(9):1745-1757. (郝怡嘉，韩雪祥*。癌症mRNA-LNP疫苗的研究进展。生命的化学, 2024, 44(9):1745-1757）[Link]

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    		2. Xuexiang Han+, Mohamad-Gabriel Alameh+, Ningqiang Gong+, Lulu Xue+, Majed Ghattas, Goutham Bojja, Junchao Xu, Gan Zhao, Claude C. Warzecha, Marshall S. Padilla, Rakan El-Mayta, Garima Dwivedi, Ying Xu, Andrew E. Vaughan, James M. Wilson, Drew Weissman*, Michael J. Mitchell*. Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo. Nature Chemistry, 2024, 16, pages1687–1697. [Link]

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    1. Xuexiang Han+, Junchao Xu+, Ying Xu, Mohamad-Gabriel Alameh, Lulu Xue, Ningqiang Gong, Rakan El-Mayta, Rohan Palanki, Claude C. Warzecha, Gan Zhao, Andrew E. Vaughan, James M. Wilson, Drew Weissman, Michael J. Mitchell*. In situ combinatorial synthesis of degradable branched lipidoids for systemic delivery of mRNA therapeutics and gene editors. Nature Communications, 2024, 15: 1762. [Link]

Before Joining CEMCS

    1. X. Han+, M.G. Alameh+, K. Butowska, J.J. Knox, K. Lundgreen, M. Ghattas, N. Gong, L. Xue, Y. Xu, M. Lavertu, P. Bates, J. Xu, G. Nie, Y. Zhong, D. Weissman*, M.J. Mitchell*. Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines. Nat. Nanotechnol., 2023, 18: 1105.

    2. N. Gong+, X. Han+, L. Xue+, R. El-Mayta, A.E. Metzloff, M.M. Billingsley, A.G. Hamilton, M.J. Mitchell*. In situ PEGylation of CAR T cells alleviates cytokine release syndrome and neurotoxicity. Nat. Mater., 2023, 22: 1571.

    3. X. Han+, N. Gong+, L. Xue, M.M. Billingsley, R. El-Mayta, S.J. Shepherd, M.G. Alameh, D. Weissman, M.J. Mitchell*. Ligand-Tethered Lipid Nanoparticles for Targeted RNA Delivery to Treat Liver Fibrosis. Nat. Commun.,2023, 14: 75.

    4. X. Huang+, E. Kon+, X. Han+, X. Zhang, N. Kong, M.J. Mitchell*, P. Dan*, W. Tao*. Nanotechnology-based strategies against SARS-CoV-2 variants, Nat. Nanotechnol., 2022，17: 1027-1037.

    5. K. Butowska+, X. Han+, N. Gong, R. El-Mayta, R.M. Haley, L. Xue, W. Zhong, W. Guo, K. Wang, M.J. Mitchell*. Doxorubicin-conjugated siRNA lipid nanoparticles for combination cancer therapy. Acta Pharm. Sin. B, 2023, 13: 1429-1437.

    6. H. Zhang+, X. Han+, M.G. Alameh, S.J. Shepherd, M.S. Padilla, L. Xue, K. Butowska, D. Weissman, M.J. Mitchell*. Rational design of anti-inflammatory lipid nanoparticles for mRNA delivery. J. Biomed. Mater. Res. A, 2022, 110: 1101-1108. (selected as the cover)

    7. X. Han+, H. Zhang+, K. Butowska, K.L. Swingle, M.G. Alameh, D. Weissman, M.J. Mitchell*. An Ionizable Lipid Toolbox for RNA Delivery, Nat. Commun., 2021, 12, 7233. (Top 25 most read Nat. Commun. articles in 2021)

    8. Y. Zhang+, X. Han+, G. Nie*, Responsive and activable nanomedicines for remodeling the tumor microenvironment, Nat. Protoc., 2021, 16 (1): 405-430.

    9. X. Han, M.J. Mitchell*, G. Nie*. Nanomaterials for Therapeutic RNA Delivery, Matter, 2020, 3(6): 1948-1975.

    10. X. Han+, K. Cheng+, Y. Xu+, Y. Wang, H. Min, Y. Zhang, X. Zhao, R. Zhao, G.J. Anderson, L. Ren, G. Nie*, Y. Li*, Modularly Designed Peptide Nanoprodrug Augments Anti-Tumor Immunity of PD-L1 Checkpoint Blockade by Targeting Indoleamine 2, 3-Dioxygenase, J. Am. Chem. Soc., 2020, 142: 2490-2496.

    11. X. Han+, Y. Xu+, M. Geranpayehvaghei, G.J. Anderson, Y. Li*, G. Nie*, Emerging nanomedicines for anti-stromal therapy against desmoplastic tumors, Biomaterials, 2020, 232: 119745.

    12. X. Han+, Y. Xu+, Y. Li*, X. Zhao, Y. Zhang, H. Min, G.J. Anderson, Y. Zhao*, G. Nie*, An extendable star-like nanoplatform for functional and anatomical imaging-guided photothermal oncotherapy, ACS Nano, 2019, 13(4): 4379-4391.

    13. X. Han, G. Nie*,Say no to tumors: NO matters, Matter, 2019, 1(4): 794-796.

    14. Y. Xu+, X. Han+, Y. Li*, H. Min, X. Zhao, Y. Zhang, Y. Qi, J. Shi, S. Qi, Y. Bao*, G. Nie*, Sulforaphane mediates glutathione depletion via polymeric nanoparticles to restore cisplatin chemosensitivity, ACS Nano, 2019, 13(11): 13445-13455.

    15. X. Han, Y. Li*, Y. Xu, X. Zhao, Y. Zhang, X. Yang, Y. Wang, R. Zhao, G.J. Anderson, Y. Zhao*, G. Nie*, Reversal of pancreatic desmoplasiaby re-educating stellate cells with a tumour microenvironment-activated nanosystem, Nat. Commun., 2018, 9: 3390.

    16. R. Zhao+, X. Han+, Y. Li*, H. Wang, T. Ji, Y. Zhao, G. Nie*, Photothermal effect enhanced cascade-targeting strategy for improved pancreatic cancer therapy by gold nanoshell@mesoporous silica nanorod, ACS Nano, 2017, 11(8): 8103-8113.

    17. Máté Vadovics, Wenchen Zhao, Emily F. Daley, Kieu Lam, Owen Daly, Khalid Rashid, Hailey R. Lee, Petra Schreiner, Kendall A. Lundgreen, Brian T. Gaudette, Vladimir V. Shuvaev, Evguenia Arguiri, Hiromi Muramatsu, András Sárközy, Thandiswa Mdluli, Junchao Xu, Xuexiang Han, Nina De Luna, Diana Castaño, Emily Bettini, Edit Ábrahám, Zoltan Lipinszki, Giuseppe Carlucci, Avinash Haridas Bansode, Katelyn Nguyen, Thuc M. Le, Tony Luu, Vladimir R. Muzykantov, Paul Bates, David Allman, Michael J. Mitchell, Michela Locci, Caius G. Radu, James Heyes & Norbert Pardi. Tailoring the adjuvanticity of lipid nanoparticles by PEG lipid ratio and phospholipid modifications.  Nat. Nanotechnol., 2025, https://doi.org/10.1038/s41565-025-01958-5.

    18. Kelsey L. Swingle, Alex G. Hamilton, Hannah C. Safford, Hannah C. Geisler, Ajay S. Thatte, Rohan Palanki, Amanda M. Murray, Emily L. Han, Alvin J. Mukalel, Xuexiang Han, Ryann A. Joseph, Aditi A. Ghalsasi, Mohamad-Gabriel Alameh, Drew Weissman & Michael J. Mitchell. Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia. Nature. 2025, 637, 412–421.

    19. Lulu Xue, Gan Zhao, Ningqiang Gong, Xuexiang Han, Sarah J. Shepherd, Xinhong Xiong, Zebin Xiao, Rohan Palanki, Junchao Xu, Kelsey L. Swingle, Claude C. Warzecha, Rakan El-Mayta, Vivek Chowdhary, Il-Chul Yoon, Jingcheng Xu, Jiaxi Cui, Yi Shi, Mohamad-Gabriel Alameh, Karin Wang, Lili Wang, Darrin J. Pochan, Drew Weissman, Andrew E. Vaughan, James M. Wilson & Michael J. Mitchell. Combinatorial design of siloxane-incorporated lipid nanoparticles augments intracellular processing for tissue-specific mRNA therapeutic delivery. Nat. Nanotechnol., 2025, 20,132–143.

    20. N. Gong, X. Han, L. Xue, M.M. Billingsley, X. Huang, R. El-Mayta, J. Qin, N.C. Sheppard, C.H. June, M.J. Mitchell. Small-molecule-mediated control of the anti-tumour activity and off-tumour toxicity of a supramolecular bispecific T cell engager. Nat. Biomed. Eng., 2024, 8:513-528.

    21. A.G. Hamilton, K.L. Swingle, A.S. Thatte, A.J. Mukalel, H.C. Safford, X. Han, A.E. Metzloff, M.J. Mitchell. High-Throughput In Vivo Screening Identifies Differential Influences on mRNA Lipid Nanoparticle Immune Cell Delivery by Administration Route. ACS Nano, 2024, DOI: 10.1021/acsnano.4c01171.

    22. A.E. Metzloff, M.S. Padilla, N. Gong, M.M. Billingsley, X. Han, M. Merolle, D. Mai, C.H. June, M.J. Mitchell. Antigen Presenting Cell Mimetic Lipid Nanoparticles for Rapid mRNA CAR T Cell Cancer Immunotherapy. Adv. Mater., 2024, 2313226.

    23. L. Xue, A.G. Hamilton, G. Zhao, Z. Xiao, R. El-Mayta, X. Han, N. Gong, X. Xiong, D. Weissman, M.J. Mitchell. High-throughput barcoding of nanoparticles identifies cationic, degradable lipid-like materials for mRNA delivery to the lungs in female preclinical models. Nat. Commun., 2024, 15: 1884.

    24. A.J. Mukalel, A.G. Hamilton, M.M. Billingsley, J. Li, A.S. Thatte, X. Han, D. Weissman, M.J. Mitchell*. Oxidized mRNA Lipid Nanoparticles for In Situ Chimeric Antigen Receptor Monocyte Engineering. Adv. Funct. Mater., 2024, 2312038.

    25. G. Zhao, L. Xue, A.I. Weiner, N. Gong, S. Adams-Tzivelekidis, J. Wong, X. Han, M.J. Mitchell*, A.E. Vaughan*. TGF-βR2 signaling coordinates pulmonary vascular repair after viral injury in mice and human tissue. Sci. Transl. Med., 2024, 16: eadg6229.

    26. L. Xue, A.S. Thatte, D. Mai, R.M. Haley, N. Gong, X. Han, K. Wang, N.C. Sheppard, C.H. June, M.J. Mitchell*. Responsive biomaterials: optimizing control of cancer immunotherapy.Nat. Rev. Mater, 2024, 9: 100.

    27. M.M. Billingsley, N. Gong, A.J. Mukalel, A.S. Thatte, R.E. Mayta, S.K. Patel, X. Han, D. Weissman, M.J. Mitchell*. In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism. Small, 2023: 2304378.

    28. W. Zhong, Y. Lu, X. Han, J. Yang, Z. Qin, W. Zhang, Z. Yu, B. Wu, S. Liu, W. Xu, C. Zheng, X. Xu, W. Guo*. Upregulation of exosome secretion from tumor-associated macrophages plays a key role in the suppression of anti-tumor immunity. Cell Reports, 2023, 42: 113224.

    29. S.J. Shepherd, X. Han, A.J. Mukalel, R.E. Mayta, A.S. Thatte, J. Wu, M.S. Padilla, M.G. Alameh, N. Srikumar, D. Lee, D. Weissman, D. Issadore*, M.J. Mitchell*, Throughput-scalable manufacturing of SARS-CoV-2 mRNA lipid nanoparticle vaccines, PNAS, 2023, 120 (33), e2303567120.

    30. J. Xu, N. Yan, C. Wang, C. Gao, X. Han, C. Yang, J. Xu, K. Wang, M.J. Mitchell, Y. Zhang*, G. Nie*. Platelet-Mimicking Nanosponges for Functional Reversal of Antiplatelet Agents, Circulation Research, 2023

    31. R.E. Mayta, M. Padilla, M. Billingsley, X. Han, M.J. Mitchell*. Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing. J. Vis. Exp. 2023, 191: e64810.

    32. L. Xue, N. Gong, S.J. Shepherd, X. Xiong, X. Liao, X. Han, G. Zhao, D. Weissman, M.J. Mitchell*. Rational Design of Bisphosphonate Lipid-like Materials for mRNA Delivery to the Bone Microenvironment. J. Am. Chem. Soc., 2022,, 144, 22, 9926-9937.

    33. S.K. Patel, M.M. Billingsley, C. Frazee, X. Han, K.L. Swingle, J. Qin, M.G. Alameh, K. Wang, D. Weissman, M.J. Mitchell*. Hydroxycholesterol substitution in ionizable lipid nanoparticles for mRNA delivery to T cells. J. Control. Release., 2022, 347: 521-532.

    34. H. Parhiz, J.S. Brenner, P. Patel, T.E. Papp, H. Shahnawaz, Q. Li, R. Shi, M. Zamora, A. Yadegari, O.A. Marcos-Contreras, A. Natesan, N. Pardi, V.V. Shuvaev, R. Kiseleva, J. Myerson, T. Uhler, R.S. Riley, X. Han, M.J. Mitchell, K. Lam, J. Heyes, D. Weissman, V. Muzykantov*. Added to pre-existing inflammation, mRNA-lipid nanoparticles induce inflammation exacerbation (IE). J. Control. Release., 2022, 344: 50-61.

    35. K. Cheng, R. Zhao, Y. Li, Y. Qi, Y. Wang, Y. Zhang, H. Qin, Y. Qin, L. Chen, C. Li, J. Liang, Y. Li, J. Xu, X. Han, G.J. Anderson, J. Shi, L. Ren, X. Zhao*, G. Nie*. Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology. Nat. Commun., 2021, 12(1), 1-16.

    36. H. Qin, R. Zhao*, Y. Qin, J. Zhu, L. Chen, C. Di, X. Han, K. Cheng, Y. Zhang, Y. Zhao, J. Shi, G.J. Anderson, Y. Zhao, G. Nie*. Development of a Cancer Vaccine Using In Vivo Click-Chemistry-Mediated Active Lymph Node Accumulation for Improved Immunotherapy. Adv. Mater., 2021, 33(20): 2006007.

    37. Y. Li, X. Zhao, X. Liu, K. Cheng, X. Han, Y. Zhang, H. Min, G. Liu, J. Xu, J. Shi, H. Qin, H. Fan, L. Ren, G. Nie. A Bioinspired Nanoprobe with Multilevel Responsive T1-Weighted MR Signal-Amplification Illuminates Ultrasmall Metastases. Adv. Mater., 2020, 32(4): 1906799.

    38. H. Min, Y. Qi, Y. Zhang, X. Han, K. Cheng, Y. Liu, H. Liu, J. Hu, G. Nie*, Y. Li*, A Graphdiyne oxide-based iron sponge with photothermally enhanced tumor-specific Fenton chemistry, Adv. Mater., 2020, 32(31): 2000038.

    39. X. Zhao, L Li, Y Zhao, H. An, Q. Cai, J. Lang, X. Han, B. Peng, Y. Fei, H. Liu, H. Qin, G. Nie, H. Wang*. In Situ Self-Assembled Nanofibers Precisely Target Cancer-Associated Fibroblasts for Improved Tumor Imaging. Angew. Chem., Int. Ed., 2019, 131: 15431.

    40. H. Min, J. Wang, Y. Qin, Y. Zhang,X. Han, Y. Xu, J. Xu, Y. Li, L. Chen, K. Cheng, G. Liu, Y. Li, G. Nie*. Biomimetic metal-organic framework nanoparticles for cooperative combination of antiangiogenesis and photodynamic therapy for enhanced efficacy. Adv. Mater., 2019: 1808200.

    41. J. Lang, X. Zhao, X. Wang, Y. Zhao, Y. Li, R. Zhao, K. Cheng, Y. Li, X. Han, X. Zheng, H. Qin, M. Geranpayehvaghei, J. Shi, G.J. Anderson, J. Hao, H. Ren, G. Nie*. Targeted co-delivery of the iron chelator deferoxamine and a HIF1α inhibitor impairs pancreatic tumor growth. ACS Nano, 2019, 13(2): 2176-2189.

    42. J. Lang, X. Zhao, Y. Qi, Y. Zhang, X. Han, Y. Ding, J. Guan, T. Ji, Y. Zhao, G. Nie. Reshaping Prostate Tumor Microenvironment To Suppress Metastasis via Cancer-Associated Fibroblast Inactivation with Peptide-Assembly-Based Nanosystem. ACS Nano, 2019, 13:12357.

    43. L. Zhang, Y. Qi, H. Min, C. Ni, F. Wang, B. Wang, H. Qin, Y. Zhang, G. Liu, Y. Qin, X. Duan, F. Li, X. Han, N. Tao, L. Zhang, Z. Qin, Y. Zhao, G. Nie*. Cooperatively responsive peptide nanotherapeutic that regulates angiopoietin receptor Tie2 activity in tumor microenvironment to prevent breast tumor relapse after chemotherapy. ACS Nano, 2019, 13(8): 5091-5102.

    44. H. Min, Y. Qi, Y. Chen, Y. Zhang, X. Han, Y. Xu, Y. Liu, J. Hu, H. Liu, Y. Li*, G. Nie*. Synthesis and imaging of biocompatible graphdiyne quantum dots. ACS Appl. Mater. Interfaces, 2019, 11: 32798.

    45. M. Taleb, Y. Ding, B. Wang, N. Yang, X. Han, C. Du, Y. Qi, Y. Zhang, Z.F. Sabet, H.R. Alanagh, A. Mujeeb, K. Khajeh, G. Nie*. Dopamine Delivery via pH-Sensitive Nanoparticles for Tumor Blood Vessel Normalization and an Improved Effect of Cancer Chemotherapeutic Drugs. Adv. Health. Mater., 2019, 8(18): 1900283.

    46. C. Du, Y. Qi, Y. Zhang, Y. Wang, X. Zhao, H. Min, X. Han, J. Lang, H. Qin, Q. Shi, Z. Zhang, X. Tian, G.J. Anderson, Y. Zhao, G. Nie*, Y. Yang*. Epidermal growth factor receptor-targeting peptide nanoparticles simultaneously deliver gemcitabine and olaparib to treat pancreatic cancer with breast cancer 2 (BRCA2) mutation. ACS Nano, 2018, 12(11): 10785-10796

    47. F. Li, Y. Li, X. Yang, X. Han, Y. Jiao, T. Wei, D. Yang, H. Xu, G*. Nie*, Highly fluorescent chiral N-S-doped carbon dots from cysteine: affecting cellular energy metabolism, Angew. Chem., Int. Ed., 2018, 130(9): 2401-2406.

    48. L. You, J. Wang, T. Liu, Y. Zhang, X. Han, T. Wang, S. Guo, T. Dong, J. Xu, G.J. Anderson, Q. Liu, Y.Z. Chang, X. Lou, G. Nie*, Targeted brain delivery of rabies virus glycoprotein 29-modified deferoxamine-loaded nanoparticles reverses functional deficits in parkinsonian mice, ACS Nano, 2018, 12(5): 4123-4139.

    49. X. Zhao, X. Wang, W. Sun, K. Cheng, H. Qin, X. Han, Y. Lin, Y. Wang, J. Lang, R. Zhao, X. Zheng, Y. Zhao, J. Hao, Q.R. Miao, G. Nie, H. Ren*. Precision design of nanomedicines to restore gemcitabine chemosensitivity for personalized pancreatic ductal adenocarcinoma treatment. Biomaterials, 2018, 158: 44.

    50. B. Wang, Y. Ding, X. Zhao, X. Han, N. Yang, Y. Zhang, Y. Zhao, X. Zhao, M. Taleb, Q.R. Miao, G. Nie*. Delivery of small interfering RNA against Nogo-B receptor via tumor-acidity responsive nanoparticles for tumor vessel normalization and metastasis suppression. Biomaterials, 2018, 175: 110.

    51. Y. Qi, H. Min, A. Mujeeb, Y. Zhang, X. Han, X. Zhao, G.J. Anderson, Y. Zhao, G. Nie*. Injectable hexapeptide hydrogel for localized chemotherapy prevents breast cancer recurrence. ACS Appl. Mater. Interfaces, 2018, 10: 6972.

    52. Y. Zhang, J. Wei, S. Liu, J. Wang, X. Han, H. Qin, J. Lang, K. Cheng, Y. Li, Y. Qi, G.J. Anderson, S. Sukumar, S. Li, G. Nie*. Inhibition of platelet function using liposomal nanoparticles blocks tumor metastasis. Theranostics, 2017, 7:1062.

    53. F. Li, T. Li, X. Han, H. Zhuang, G. Nie, H. Xu*. Nanomedicine assembled by coordinated selenium-platinum complexes can selectively induce cytotoxicity in cancer cells by targeting the glutathione antioxidant defense system. ACS Biomater. Sci. Eng., 2018, 4: 1954.

    54. X. Zhao, X. Wang, L. Fang, C. Lan, X. Zheng, Y. Wang, Y. Zhang, X. Han, S. Li, K. Cheng, Y. Zhao, J. Shi, J. Guo, J. Hao, H. Ren, G. Nie*. A combinatorial strategy using YAP and pan-RAF inhibitors for treating KRAS-mutant pancreatic cancer. Cancer letters, 2017, 402:61.

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