• Target Gene (KI Gene): Srcap GTP CODE: 18000050
• KI Locus: C-terminal KI Tag: HA
• NCBI Reference: NM_001304266.1 Tag Sequence:

• KI Donor Plasmid 1: pT-Srcap-C-HA Plasmid 1 Sequence:
• KI Donor Plasmid 2: Srcap-C-sg sgRNA Sequence: CGAAAGGCCAAGACGTGAAT

• Western Blot
• Protein expression in AG-haESCs: +++ ++ + -   (+++/++/+/-)

• 基因型鉴定文件

• Reference:Ye, B., Yang, L., Qian, G., Liu, B., Zhu, X., Zhu, P., Ma, J., Xie, W., Li, H., Lu, T., et al. (2020). The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells. The EMBO journal 39, e103786.