Tagged AG-haESCs

Protein Tag

Basic Information
  • Target Gene (KI Gene): Tuba1a GTP CODE: 19000774
  • KI Locus: N-terminal KI Tag: EGFP
  • NCBI Reference: NM_011653.2 Tag Sequence:


Plasmid Information
  • KI Donor Plasmid 1: pT-Tuba1a-N-EGFP/pT-Tuba1c-N-mCherry Plasmid 1 Sequence:
  • KI Donor Plasmid 2: Tuba1a-N-sg/Tuba1c-N-sg sgRNA Sequence: ACCCTCTATCTACCGAGAGC/TAGCGGCGAGGTCGTCTGAA


Western Blot Result
  • Western Blot 152x200
  • Protein expression in AG-haESCs:   (+++/++/+/-)
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