• Target Gene (KI Gene): Fxr1 GTP CODE: 18000128
• KI Locus: N-terminal KI Tag: Flag
• NCBI Reference: NM_001113188.1 Tag Sequence:

• KI Donor Plasmid 1: pT-Fxr1-N-3F Plasmid 1 Sequence:
• KI Donor Plasmid 2: Fxr1-N-SG sgRNA Sequence: CCTTTGCGGTTCCAACATGG

• Western Blot
• Protein expression in AG-haESCs: +++ ++ + -   (+++/++/+/-)

• Genotype File

• Reference:Kang, J. Y., Wen, Z., Pan, D., Zhang, Y., Li, Q., Zhong, A., Yu, X., Wu, Y. C., Chen, Y., Zhang, X., et al. (2022). LLPS of FXR1 drives spermiogenesis by activating translation of stored mRNAs. Science 12, 377(6607), eabj6647.