Home CAS Center for Excellence


Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
LIU Xiaolong
Ph.D., Professor
Director of IBCB
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: liux@@sibs.ac.cn

Research Areas
thymocyte; TCR gene recombination; CD4/CD8 lineage differentiation; T cell activation and function; Tanscription Factors; DNA methylation; histone acetylation; non-coding RNA; Transgenic and knockout mice

Research Interests
My lab is interested in the molecular mechanisms of thymocyte development, including (1) how the ordering and allelic exclusion of T cell receptor (TCR) gene recombination are achieved, (2) how TCR signaling and its downstream effectors control positive/negative selection and (3) how transcription factors determine the CD4/CD8 lineage commitment. We are also interested in how peripheral T cells collaborate with other immune cells to control immune response. Currently, our research approaches include biochemical, molecular biological and immunological techniques, large-scale gene expression profiling and analysis of transgenic and knockout mice.

Selected Publications

  1. Xu Y, Sun Y, Shen H, Dai Y, Liu H, Li R, Zhang H, Wu L, Zhu X, Liu X*. Regulation of the terminal maturation of iNKT cells by mediator complex subunit 23. Nat Commun. 2018; 9(1):3875.
  2. Chen X, Zhao J, Gu C, Cui Y, Dai Y, Song G, Liu H, Shen H, Liu Y, Wang Y, Xing H, Zhu X, Hao P, Guo F*, Liu X*. Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells. Nat Commun. 2018; 9(1):3746.
  3. Zhang R, Ma H, Gao Y, Wu Y, Qiao Y, Geng A, Cai C, Han Y, Zeng YA, Liu X*, Ge G*. Th-POK regulates mammary gland lactation through mTOR-SREBP pathway. PLoS Genet. 2018; 14(2):e1007211.
  4. Zhao J, Chen X, Song G, Zhang J, Liu H, Liu X*. Uhrf1 controls the self-renewal versus differentiation of hematopoietic stem cells by epigenetically regulating the cell-division modes. Proc Natl Acad Sci U S A. 2017; 114(2):E142-E151. [Highlighted by Proc Natl Acad Sci U S A. 2017; 114(2):192-94].
  5. Chen J, Cai Z, Zhang L, Yin Y, Chen X, Chen C, Zhang Y, Zhai S, Long X, Liu X*, Wang X*. Lis1 Regulates Germinal Center B Cell Antigen Acquisition and Affinity Maturation. J Immunol. 2017;198(11):4304-4311.
  6. Cui Y, Chen X, Zhang J, Sun X, Liu H, Bai L, Xu C, Liu X*. Uhrf1 Controls iNKT Cell Survival and Differentiation through the Akt-mTOR Axis. Cell Rep. 2016; 15(2):256-63.
  7. Xu K, Liu H, Shi Z, Song G, Zhu X, Jiang Y, Zhou Z, Liu X*. Disruption of the RAG2 zinc finger motif impairs protein stability and causes immunodeficiency. Eur J Immunol. 2016; 46(4):1011-9.
  8. Deng Z, Liu H, Rui J, Liu X*. Reconstituted Thymus Organ Culture. Methods Mol Biol. 2016; 1323:151-8.
  9. Deng Z, Liu H, Liu X*. RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination. Cell Res. 2015; 25(2):181-92.
  10. Sun Y, Zhu X, Chen X, Liu H, Xu Y, Chu Y, Wang G*, Liu X*. The mediator subunit Med23 contributes to controlling T-cell activation and prevents autoimmunity. Nat Commun. 2014; 5:5225.
  11. Zhang Y, Xu K, Deng A, Fu X, Xu A*, Liu X*. An amphioxus RAG1-like DNA fragment encodes a functional central domain of vertebrate core RAG1. Proc Natl Acad Sci U S A. 2014; 111(1):397-402.
  12. Chen X, Zhang J, Zhao J, Liu H, Sun X, Zhao M, Liu X*. Lis1 is required for the expansion of hematopoietic stem cells in the fetal liver. Cell Res. 2014; 24:1013-16.
  13. Xiao G, Deng A, Liu H, Ge G, Liu X*. Activator protein 1 suppresses antitumor T-cell function via the induction of programmed death 1. Proc Natl Acad Sci U S A. 2012; 109(38):15419-24.
  14. Rui J, Liu H, Zhu X, Cui Y, Liu X*. Epigenetic silencing of CD8 genes by ThPOK-mediated deacetylation during CD4 T cell differentiation. J Immunol. 2012; 189(3):1380-90. [Highlighted by J Immunol. 2012; 189(3):1098].
  15. Cao Y, Li H, Liu H, Zhang M, Hua Z, Ji H, Liu X*. LKB1 regulates TCR-mediated PLCγ1 activation and thymocyte positive selection. EMBO J. 2011; 30(10):2083-93. [Selected by Faculty of 1000 Biology]
  16. Wen X, Liu H, Xiao G, Liu X*. Downregulation of the transcription factor KLF4 is required for the lineage commitment of T cells. Cell Res. 2011; 21(12):1701-10.
  17. Zhang M, Zhang J, Rui J, Liu X*. p300-Mediated Acetylation Stabilizes the Th-Inducing POK Factor. J Immunol. 2010; 185(7):3960-9. [Highlighted by J Immunol. 2010; 185(7):3783]
  18. Cao Y, Li H, Sun Y, Chen X, Liu H, Gao X and Liu X*. Interferon regulatory factor 4 regulates thymocyte differentiation by repressing Runx3 expression. Eur J Immunol. 2010; 40(11):3198-209.
  19. Cao Y, Li H, Liu H, Zheng C, JI H, Liu X*. The serine/threonine kinase LKB1 controls thymocyte survival by regulation of AMPK activation and Bcl-XL expression. Cell Res. 2010; 20:99-108.
  20. Zhang Y, Li H, Wang X, Gao X, Liu X*. Regulation of T cell development and activation by creatine kinase B. PLoS One. 2009; 4(4):e5000.
  21. Yang Z, Cao Y, Zhu X, Huang Y, Ding Y, Liu X*. Znhit1 causes cell cycle arrest and down-regulates CDK6 expression. Biochem Biophys Res Commun. 2009; 386(1):146-52.
  22. Wang X, Zhang Y, Xiao G, Gao X, Liu X*. c-Fos enhances survival of thymocytes during positive selection by upregulating Bcl-2. Cell Res. 2009; 19:340-347.
  23. Wang X, Xiao G, Zhang Y, Wen X, Gao X, Okada S, Liu X*. Regulation of Tcrb recombination ordering by c-Fos-dependent RAG deposition. Nat Immunol. 2008; 9(7):794-801. [Research highlighted by Nat Rev Immunol. 2008; 8(7):494]
  24. Zhang Y, Yang Z, Cao Y, Zhang S, Li H, Huang Y, Ding YQ, Liu X*. The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell cycle arrest in T cells. Biochem J. 2008; 413(2):239-250.
  25. Shi J, Yang Z, Wang M, Cheng G, Li D, Wang Y, Zhou Y, Liu X*, Xu C*. Screening of an antigen target for immunocontraceptives from cross-reactive antigens between human sperm and Ureaplasma urealyticum. Infect Immun. 2007; 75(4):2004-2011.
  26. Sun G, Liu X, Mercado P, Jenkinson SR, Kypriotou M, Feigenbaum L, Gal└ra P, Bosselut R*. The zinc finger protein cKrox directs CD4 lineage differentiation during intrathymic T cell positive selection. Nat Immunol. 2005; 6(4):373-381.
  27. Liu X, Taylor BJ, Sun G, Bosselut R*. Analyzing expression of perforin, Runx3, and Thpok genes during positive selection reveals activation of CD8-differentiation programs by MHC II-signaled thymocytes. J Immunol. 2005; 175(7):4465-4474.
  28. Liu X, Bosselut R*. Duration of TCR signaling controls CD4-CD8 lineage differentiation in vivo. Nat Immunol. 2004; 5(3):280-288.
  29. Liu X, Adams A, Wildt KF, Aronow B, Feigenbaum L, Bosselut R*. Restricting Zap70 expression to CD4+CD8+ thymocytes reveals a T cell receptor-dependent proofreading mechanism controlling the completion of positive selection. J Exp Med. 2003; 197(3):363-373.
  30. Liu X, Wu X and Zhou Y*. Identification of key residues responsible for enzymatic and platelet-aggregation-inhibiting activities of acidic phospholipase A2 from Agkistrodon halys Pall. J Nat Toxins. 2001; 10: 43-55.
  31. Liu X, Xiong Y, Wu X and Zhou Y*. A comparative study on the structure and function of phospholipase A2s from Agkistrodon acutus. Protein Peptide Lett. 2000; 7: 83-90.
  32. Liu X, Pan H., Yang G, Wu X and Zhou Y*. Cloning, Expression and Biochemical Characterization of A Basic-Acidic Hybrid Phospholipase A2-II From Agkistrodon halys Pallas. Biochim Biophys Acta. 1999; 1431: 157-165.

Education Background & Academic Experience
Feb 2004: Principle Investigator, Shanghai Institute of Biochemistry and Cell Biology/SIBS
Oct 1999-Jan 2004: Postdoctor, Visiting Fellow, National Cancer Institute/NIH
Sep 1994-Jun 1999: Ph.D Candidate, Shanghai Institute of Biochemistry/CAS
Sep 1990-July 1994: College Student, Nanchang University

Research Team


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