Home CAS Center for Excellence
  
  
  

Faculty

Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
 
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
 
Principal Investigators
 
LI Jinsong
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: jsli@@sibcb.ac.cn

Research Areas
Cellular reprogramming; Nuclear transfer; Haploid ESCs; Semi-cloned technology; Primordial germ cell development; Spermatogonial stem cells; Disease modeling

Research Interests

In 2012, we reported that the generation of androgenetic haploid embryonic stem cells (AG-haESCs) that can support full-term embryonic development upon injection into MII oocytes, leading to the generation of semi-cloned (SC) mice (Yang et al., Cell, 2012). However, one major drawback of this study is the frequently observed aberrant development of AG-haESC-derived embryos and the very low birth rate of healthy SC mice (around 2% of total SC embryos); this greatly restricts the applications of AG-haESCs. In 2015, we showed that AG-haESCs carrying deletions in the DMRs (differentially DNA methylated regions) controlling two paternally repressed imprinted genes, H19 and Gtl2, designated as DKO-AG-haESCs or ‘artificial spermatids’, can efficiently support the generation of SC pups at a rate of 20% (Zhong et al., Cell Stem Cell, 2015). Recently, through combining DKO-AG-haESCs and CRISPR-Cas9-mediated base editor system, we showed that mice with different base mutations can be generated in one step, enabling identification of critical amino acids of DND1 for primordial germ cell (PGC) development (Li et al., Nature Cell Biology, 2018). 
Currently, we focus on the development of primordial germ cells, generation of disease models and large-scale tagging proteins in mice through ‘artificial spermatid’-mediated semi-cloned technology.
1. Identification of critical factors involved in PGC development and revealing underlying mechanisms.
2. Generation of disease models that mimic complex syndromes and understanding the pathological mechanisms.
3. Tagging every protein in mice (genome tagging project, GTP) and study of protein network during different germ cell development.
4. Study of underlying mechanisms of stemness and differentiation of spermatogonial stem cells.

Selected Publications

(#Co-first authors, *: Correspondence authors)
  1. Li Y & Li J*. Technical advances contribute to the study of genome imprinting. PLOS Genet 2019. (Invited review)
  2. Wang L & Li J*. ‘Artificial spermatid’-mediated genome editing. Biol Reprod 2019. (Invited review)
  3. Wang J#, Chen J#, Wu G#, Zhang H#, Du X#, Chen S, Zhang L, Wang K, Fan J, Gao S, Wu X, Zhang S, Kuai B, Zhao P, Chi B, Wang L, Li G, Wong CCL, Zhou Y*, Li J*, Yun C* & Cheng H*. NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction. Genes & Dev 2019 Mar 10. DOI: 10.1101/gad.322602.118
  4. Song W, Wang R, Jiang W, Yin Q, Peng G, Yang R, Yu QC, Chen J, Li J, Cheung TH, Jing N & Zeng Y. Hormones induce the formation of luminal-derived basal cells in the mammary gland. Cell Res 2019 Jan 10.
  5. Li J* & Gao C*. Preface to the special topic on genome editing research in China. Nat Sci Rev 2019
  6. Jiang Jing, Yan M, Li D & Li J*. Genome tagging project: tag every protein in mouse through “artificial spermatids”. Nat Sci Rev 2019
  7. Lander E, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li J, Liu D, Naldini L, Nie JB, Qiu R, Schoene-Seifert B, Shao F, Terry S, Wei W & Winnacker EL. Adopt a moratorium on heritable genome editing. Nature 567(7747)(2019): 165-168.
  8. Wang Haoyi, Li J, Li W, Gao C & Wei W. CRISPR twins: China Academy responds. Nature 564(7736)(2018): 345.
  9. Li Qing, Li Y, Yang S, Huang S, Yan M, Ding Y, Tang W, Lou X, Yin Q, Sun Z, Lu L, Shi H, Wang H, Chen Y* & Li J*. CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol 20(11)(2018): 1315-1325. Epub 2018 Oct 01.
  10. Chen Yao, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J*, Tang F* & Tong M*. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Res 28(9)(2018), 879-896. Epub 2018 Jul 30.
  11. Qu Chao, Yan M, Yang S, Wang L, Yin Q, Liu Y, Chen Y & Li J*. Haploid embryonic stem cells can be enriched and maintained by simple filtration. J Biol Chem 293 (14) (2018): 5230-5235.
  12. Li ZiYi, Huang M, Wang X, Zhu Y, Li J, Wong CCL & Fang Q. Nanoliter-scale oil-air-droplet chip-based single cell proteomic analysis. Anal Chem 90(8)(2018), 5430-5438. Epub 2018 Mar 27.
  13. Liu Wuqing, Li S, Zhang Y, Li J & Li Y. Efficient CRISPR-based genome editing using tandem guide RNAs and editable surrogate reporters. FEBS Open Bio 8(7)(2018), 1167-1175.
  14. Wang Xiukun & Li J*. An intermediate cell state allows rerouting of cell fate. J Biol Chem 292(46)(2017), 19133-19134. (Highlight).
  15. Wei Leixin, Wang X, Yang S, Yuan W* & Li J*. Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene-editing. J Genet Genomics 44(9)(2017), 461-463. Aug 12.
  16. Zheng Yi, Jongejan A, Mulder CL, Mastenbroek S, Repping S, Wang Y, Li J & Hamer G. Trivial role for NSMCE2 during in vitro proliferation and differentiation of male germline stem cells. Reproduction 154(3)(2017), 81-95.
  17. Li Haisen, Guo A, Xie Z, Tu W, Yu J, Wang H, Zhao J, Zhong C, Kang J, Li J, Huang S & Shen L. Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation. Sci Rep 7(1)(2017), 13222.
  18. Feng Su, Xing C, Shen T, Qiao Y, Wang R, Chen J, Liao J, Lu Z, Yang X, Abd-Allah SM, Li J, Jing N & Tang K. Abnormal paraventricular nucleus of hypothalamus and growth retardation associated with loss of nuclear receptor gene COUP-TFII. Sci Rep 7(1)(2017), 5282.
  19. Wang Yinghua, Ding Y & Li J*. CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Methods Mol Biol 1622(2017), 293-305.
  20. Zuo Erwei#, Cai Y-J#, Li K#, Wei Y#, Wang B-A#, Sun Y, Liu Z, Liu J, Hu X, Wei W, Huo X, Shi L, Tang C, Liang D, Wang Y, Nie Y-H, Zhang C-C, Yao X, Wang X, Zhou C, Ying W, Wang Q, Chen R-C, Shen Q, Xu G-L, Li J, Sun Q*, Xiong Z-Q* & Yang H*. One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs. Cell Res 27(7)(2017), 933-945. Epub 2017 June 6.
  21. Gou Lantao, Kang J, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua M, Lu Y, Zhu Y, Li Z, Chen H, Wu L, Li D, Fu X, Li J, Shi H* & Liu M*. Ubiquitination-deficient mutations in human Piwi cause male infertility by impairing histone-to-protamine exchange during spermiogenesis. Cell 169(6)(2017), 1090-1104. Epub 2017 May 25.
  22. Wu Keliang#, Zhong C#, Chen T#, Zhang X, Tao W, Zhang J, Li H, Zhao H*, Li J* & Chen ZJ*. Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy. Cell Res 27(8)(2017), 1069-1072. Epub 2017 May 12.
  23. Wang Lingbo#, Li MY#, Qu C, Miao WY, Yin Q, Liao J, Cao HT, Huang M, Wang K, Zuo E, Peng G, Zhang SX, Chen G, Li Q, Tang K, Yu Q, Li Z, Wong C, Xu G, Jing N, Yu X* & Li J*. CRISPR-Cas9-mediated genome-editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development. Cell Res 27(6)(2017), 815-829. Epub 2017 April 21.
  24. Xu He, Yue C, Zhang T, Li Y, Guo A, Liao J, Pei G, Li J* & Jing N*. Derivation of haploid neurons from mouse androgenetic haploid embryonic stem cells. Neurosci Bull 33(3)(2017), 361-364. Epub Mar 3.
  25. Wu Keliang, Chen T, Huang S, Zhong C, Yan J, Zhang X, Li J, Gao Y & Chen ZJ. Mitochondrial replacement by pre-pronuclear transfer in human embryos. Cell Res 27(6)(2017), 834-837. Epub 2017 April 4.
  26. Zhong Cuiqing & Li J*. Efficient generation of gene-modified mice by haploid embryonic stem cell-mediated semi-cloned technology. Methods Mol Biol 1498 (2017), 121-133.
  27. Wang Xiongjun, Qiao Y, Xiao MM, Wang L, Chen J, Lv W, Xu L, Li Y, Wang Y, Tan MD, Huang C, Li J, Zhao TC, Hou Z, Jing N & Chin YE. Opposing Roles of Acetylation and Phosphorylation in LIFR-Dependent Self-Renewal Growth Signaling in Mouse Embryonic Stem Cells. Cell Rep 18(4)(2017), 933-946.
  28. Yang Jiao, Guo R, Wang H, Ye X, Zhou Z, Dan J, Wang H, Gong P, Deng W, Yin Y, Mao S, Wang L, Ding J, Li J, Keefe DL, Dawlaty MM, Wang J, Xu G & Liu L. Tet enzymes regulate telomere maintenance and chromosomal stability of mouse ESCs. Cell Rep 15(8)(2016), 1-13. May 24, 2016.
  29. Jin Lifang & Li J. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research 37(4)(2016), 205-213.
  30. Burgress Shawn, Cheng L, Gu F, Huang L, Huang Z, Lin S, Li J, Li W, Qin W, Sun Y, Songyang Z, Wei W, Wu Q, Wang H, Wang X, Xiong JW, Xi J, Yang H, Zhou B & Zhang B. Questions about NgAgo. Protein Cell 7(12)(2016), 913-915.
  31. Zhong C #, Zhang M#, Yin Q, Zhao H, Wang Y, Huang S, Tao W, Wu K*, Chen Z-J* & Li J*. Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus. Cell Res 26(6)(2016), 743-746. Epub 2016 May 17.
  32. Bai M, Liang D, Wang Y, Li Q, Wu Y & Li J*. Spermatogenic cell-specific gene mutation in mice via CRISPR-Cas9. J Genet Genomics 43(5)(2016), 289-296. Epub 2016 Feb 26.
  33. Bai M, Wu Y & Li J*. Generation and application of mammalian haploid embryonic stem cells. J Intern Med 280(3)(2016), 236-245. (Invited Review).
  34. Zhong C#, Xie Z#, Yin Q#, Dong R, Yang S, Wu Y & Li J*. Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection. Cell Res 26(1)(2016), 131-134. Epub 2015 Nov 17.
  35. Zhong C#, Yin Q#, Xie Z#, Bai M#, Dong R#, Tang W, Xing Y, Zhang H, Yang S, Chen L-L, Bartolomei MS, Ferguson-Smith A, Li D, Yang L*, Wu Y* & Li J*. CRISPR-Cas9-mediated genetic screening in mice with haploid embryonic stem cells carrying a guide RNA library. Cell Stem Cell 17(2)(2015), 221-232.
  36. Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn J, Doudna J, Feng G, Greely HT, Isasi R, Ji W, Kim J, Knoppers B, Lanphier E, Li J, Lovell-Badge R, Martin GS, Moreno J, Naldini L, Pera M, Perry A, Venter JC, Zhang F & Zhou Q. CRISPR germline engineering-the community speaks. Nat Biotechnol 33(5)(2015), 478-486. (Feature).
  37. Tong M* & Li J*. Similarity of epigenetic reprogramming in primordial germ cells between human and mouse. Natl Sci Rev 2015, 2(4)(2015), 384. (Invited Research Highlights)
  38. Liu P, Dou X, Liu C, Wang L, Xing C, Peng G, Chen J, Yu F, Qiao Y, Song L, Wu Y, Yue C, Li J, Han J, Tang K & Jing N. Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. Nat Commun 6(2015), 6830.
  39. Qin Y, Qin J, Zhou C, Li J & Gao W. Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer. Cell Cycle 14(8)(2015), 1282-1290.
  40. Zhu W, Yao X, Liang Y, Liang D, Song L, Jing N, Li J & Wang G*. Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling. Development 142(3)(2015), 465-476. Epub Jan 6. pii: dev.112946.
  41. Wu Y#, Zhou H#, Fan X#, Zhang Y#, Zhang M#, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L*, Tang F* & Li J*. Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Res 25(1)(2015), 67-79. Epub 2014 Dec 5.
  42. Li J*. Stem cell, basis and application. Sci Bull 60(20)(2015), 1711-1712. (组织专刊并撰写序言).
  43. 李劲松. 基因编辑技术—后基因组时代生命科学研究的助力器. 生命科学(基因编辑专刊) 27(1)(2015), 1. (组织专刊并撰写序言)
  44. Lian X, Xu J, Li J & Chien K. Next-generation models of human cardiogenesis via genome editing. Cold Spring Harb Perspect Med 4(12)(2014), a013920. doi: 10.1101/cshperspect.a013920.
  45. Guo F#, Li X#, Liang D#, Li T#, Zhu P, Guo H, Wu X, Wen L, Gu T, Hu B, Walsh CP, Li J*, Tang F* & Xu G*. Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote. Cell Stem Cell 15(4)(2014), 447-458. [Epub ahead of print].
  46. Zhang M, Zhou H, Zheng C, Xiao J, Zuo E, Liu W, Xie D, Shi Y, Wu C, Wang H, Li D & Li J*. The roles of testicular c-kit positive cells in de novo morphogenesis of testis. Sci Rep 4(2014), 5936. doi: 10.1038/srep05936.
  47. Zhu Q, Song L, Peng G, Sun N, Chen J, Zhang T, Sheng N, Tang W, Qian C, Qiao Y, Tang K, Han JD, Li J & Jing N. The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. Elife 14(2014), e02224. doi: 10.7554/eLife.02224. [Epub ahead of print].
  48. 周海,伍春莲*,李劲松*. 不同年龄小鼠精原干细胞系的建立. 中国细胞生物学学报 36(6)(2014), 752-757.
  49. Zhao B, Yang D, Jiang J, Li J, Fan S, Huang M, Fan Y, Jin Y & Jin Y. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies. BMC Genomic 15(2014), 488. doi: 10.1186/1471-2164-15-488.
  50. Yu Y, Liang D, Tian Q, Chen X, Jiang B, Chou BK, Hu P Cheng L, Gao P, Li J & Wang G. Stimulation of somatic cell reprogramming by Eras-Akt-Foxo1 signaling axis. Stem Cells 32(2)(2014), 349-363. doi: 10.1002/stem.1447. [Epub ahead of print].
  51. Qin Y, Zhou P, Zhou C, Li J & Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev 23(4)(2014), 363-371.
  52. Wu Y#, Liang D#, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D & Li J*. Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell 13(6)(2013), 659-662.
  53. Yang H#, Liu Z#, Ma Y#, Zhong C, Yin Q, Zhou C, Shi L, Cai Y, Zhao H, Wang H, Tang F, Wang Y, Zhang C, Liu X, Lai D, Jin Y*, Sun Q* & Li J*. Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. Cell Res 23(10)(2013), 1187-1200.
  54. Qin Y#, Lin J#, Zhou C#, Yin Q, Xie Z, Zhang X, Liu X, Gao W & Li J*. Mice cloned from white adipose tissue-derived cells. J Mol Cell Biol 5(5)(2013), 348-350.
  55. Jiang J#, Lv W#, Ye X, Wang L, Zhang M, Yang H, Okuka M, Zhou C, Zhang X, Liu L* & Li J*. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Res 23(1)(2013), 92-106.
  56. Zhao S#, Gou LT#, Zhang M#, Zu LD, Hua MM, Hua Y, Shi HJ, Li Y, Li J, Li DS, Wang ED* & Liu M. piRNA-triggered MIWI ubiquitination and removal by APC/C in late spermatogenesis. Dev Cell 24(1)(2013), 13-25.
  57. Shi L, Yang H & Li J*. Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses. Protein & Cell 3(11)(2012), 806-810. (Invited review)
  58. Yang H#, Shi L#, Wang B#, Liang D, Zhong C, Liu W, Nie Y, Liu J, Zhao J, Gao X, Li D, Xu G-L* & Li J*. Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. Cell 149(3)(2012), 605-617.
  59. Ma Yu, Gu J, Li C, Wei X, Tang F, Shi G, Jiang J, Kuang Y, Li J, Wang Z, Xie X & Jin Y. Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells. Stem Cell Res Ther 3(4)(2012), 29.
  60. Gu T#, Guo F#, Yang H#, Wu HP, Xu GF, Liu W, Xie ZG, Shi L, He X, Jin SG, Iqbal K, Shi YG, Deng Z, Szabo PE, Pfeifer GP, Li J* & Xu GL*. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477(7366)(2011), 606-610.
  61. Jiang J#, Ding G#, Lin J#, Zhang M, Shi L, Lv W, Yang H, Xiao H, Pei G, Li Y, Wu J* & Li J*. Different developmental potential of pluripotent stem cells generated by different reprogramming strategies. J Mol Cell Biol 3(3)(2011), 197-199.
  62. Lin J, Shi L, Zhang M, Yang H, Qin Y, Zhang J, Gong D, Zhang X, Li D & Li J*. Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer. Cell Stem Cell 8(4)(2011), 371-375.
  63. Yang H#, Shi L#, Chen D & Li J*. Mice generated after round spermatid injection into haploid two-cell blastomeres. Cell Res 21(5)(2011), 854-858.
  64. Li X, Zhu L, Yang A, Lin J, Tang F, Jin S, Wei Z, Li J & Jin Y. Calcineurin-NFAT signaling critically regulates early lineage specification in mouse embryonic stem cells and embryos. Cell Stem Cell 8(1)(2011), 46-58.
  65. Wang Y, Chen J, Hu J, Wei X, Qin D, Gao J, Zhang L, Jiang J, Li J, Liu J, Lai K, Kuang X, Zhang J, Pei D & Xu G. Reprogramming of mouse and human somatic cells by high-performance engineered factors. EMBO Rep 12(4)(2011), 373-378.
  66. Yang H, Shi L, Zhang S, Lin J, Jiang J & Li J*. High-efficiency somatic reprogramming induced by intact MII oocytes. Cell Res 20(9)(2010), 1034-1042.
  67. Li L, Sun L, Gao F, Jiang J, Li C, Gu J, Wei Z, Yang A, Lu R, Ma Y, Tang F, Kwon SW, Zhao Y, Li J & Jin Y. Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation. Proc Natl Acad Sci USA 107(4)(2010),1402-1407.
  68. Chen T, Yuan D, Wei B, Jiang J, Kang J, Ling K, Gu Y, Li J, Xiao L & Pei G. E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation. Stem Cells 28(8)(2010), 1315-1325.
  69. Zhu Z, Wang Y, Li X, Wang Y, Xu L, Wang X, Sun T, Dong X, Chen L, Mao H, Yu Y, Li J, Chen PA & Chen CD. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis. Cell Res 20(7)(2010), 794-801.
  70. Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, Yang Y, Jin S, Wei Z, Jiang H, Li J & Jin Y. Germline-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor. PLos One 4(8)(2009), e6724.
  71. Huang J, Chen T, Liu X, Jiang J, Li J, Li D, Liu X, Li W, Kang J & Pei G. More synergetic cooperation of Yamanaka factors in induced pluripotent stem cells than in embryonic stem cells. Cell Res 19(10)(2009), 1127-1138.
  72. Li J & Mombaerts P. Nuclear transfer-mediated rescue of the nuclear genome of nonviable mouse cells frozen without cryoprotectant. Biol Reprod 79(4)(2008), 588-593.
  73. Li J#, Guasch G#, Greco V#, Fuchs E* & Mombaerts P*. Cloned mice from Skin Stem Cells. Proc Natl Acad Sci USA 104(8)(2007), 2738-2743.
  74. Li J, Ishii T, Wen D & Mombaerts P. Non-equivalence of cloned and clonal mice. Curr Biol 15(18)(2005), R756-R757. 
  75. Li J, Ishii T, Feinstein P & Mombaerts P. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981)(2004), 393-398.
  76. Han Z, Chen D, Li J, Sun Q, Wan Q, Kou Z, Rao G, Lei L, Liu Z & Fang S.  Mitochondrial DNA heteroplasmy in calves cloned by using adult somatic cell. Mol Reprod Dev 67(2)(2004), 207-214
  77. Chen D#, Li J#, Han Z, Lei L, Liu Z, Kou Z, Ma S, Du Q & Sun Q. Somatic cell bovine cloning: efficiency of donor cells from female and male using different recipient cows. Chin Sci Bull 48(6)(2003), 549-554.
  78. Zhu Z, Chen D, Li J, Lian L, Lei L, Han Z & Sun Q. Rotation of meiotic spindle is controlled by microfilaments in mouse oocytes. Biol Reprod 68(3)(2003): 943-946.
  79. Han Z, Chen D, Li J, Sun Q, Wang P, Du J & Zhang H. Flow cytometric cell-cycle analysis of cultured fibroblasts from the giant panda, Ailuropoda melanoleuca L. Cell Biol Int 27(4)(2003), 349-353.
  80. Wen D, Yang C, Cheng Y, Li J, Liu Z, Sun Q, Zhang J, Lei L, Wu Y, Kou Z & Chen D. Comparison of developmental capacity for intra- and interspecies cloned cat (Felis catus) embryos. Mol Reprod Dev 66(1)(2003), 38-45.
  81. Chen Y, He Z, Liu A, Wang K, Mao W, Chu J, Lu Y, Fang Z, Shi Y, Yang Q, Chen D, Wang M, Li J, Huang S, Kong X, Shi Y, Wang Z, Xia J, Long Z, Xue Z, Ding W & Sheng H. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes. Cell Res 13(4)(2003), 251-263.
  82. Li J, Chen D, Han Z, Zhu Z, Wen D, Liu Z, Wang M, Lian L, Du J, Wang P & Zhang H. Serial Nuclear Transfer Improves the Development of Interspecies Reconstructed Giant Panda Embryos. Chin Sci Bull 47(6)(2002), 467-469.
  83. Chen D, Wen D, Zhang Y, Sun Q, Han Z, Liu Z, Shi P, Li J, Xianyu J, Lian L, Kou Z, Wu Y, Chen Y, Wang P & Zhang H. Interspeices implantation and mitochondria fate of panda-rabbit cloned embryos. Biol Reprod 67(2)(2002), 637-642.
  84. Li J & Chen D. Cell cycle interactions between donor nucleus and recipient cytoplasm in mammalian nuclear transfer. Progress in Biochemistry and Biophysics 29(2) (2002), 206-210. (中文综述)
  85. Han Z, Chen D, Li J, Sun Q, Wang P, Huang Y & Du J. The culture of fibroblasts from diaphragm of Giant Panda. In Vitro Cell Dev Biol Anim 37(10)(2001), 644-645.
  86. Li G, Chen D, Lian L, Sun Q, Wang M, Liu J, Li J & Han Z. Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplamic sperm injection (ICSI). Mol Reprod Dev 58(2)(2001), 180-185.
  87. Ding B, Shi P, Xianyu J, Zhang Y, Chen D, Sun Q, Li G, Wang M, Liu J, Han Z, Song X, Li J & Chen Y. Microsatellite DNA analysis prove nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda. Chin Sci Bull 45(20)(2000), 1883-1885.
  88. Li J, Zhuang D, Sun Q & Chen D. New advances in animal transgenic technology. Progress in Biochemistry and Biophysics 27(2) (2000), 124-126. (中文综述)
  89. Liu J, Wang M, Lian L, Li J, Gao S, Han Z, Sun Q, Song X, Zhang D, Li Y, Xu Z & Chen D. Nuclear Transfer Using Nonquiescent Adult Fibroblasts from a Bovine Ear. Chin Sci Bull 44(21)(1999), 1971-1974.
  90. Chen D, Sun Q, Liu J, Li G, Lian L, Wang M, Han Z, Song X, Li J, Sun Q, Chen Y, Zhang Y & Ding B. The Giant Panda Somatic Nucleus can dedifferentiate in Rabbit Ooplasm and Support Early Development of the Reconstructed Egg. Sci China C Life Sci 42(4)(1999), 346-353

Education Background & Academic Experience
2002-2007: Postdoctoral Fellow, The Rockefeller University
1999-2002: Ph.D., Institute of Zoology, Chinese Academy of Sciences
1993-1996: M Sc, Yangzhou University
1989-1993: B.S., Jiangxi Agriculture University

Research Team

 

Copyright © 2017-2020 Shanghai Institute of Biochemistry and Cell Biology, CAS. All rights reserved