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Faculty

Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
 
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
 
Principal Investigators
 
CHEN Zhengjun
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: zjchen@@sibcb.ac.cn

Research Areas
cell polarity; migration; proliferation;Par3-Par6-aPKC; mLgl-1(Hugl-1); Cdc42;tumorigenesis; protein kinases; phosphoprotein proteomics; Csk-binding protein; CASK; insulin; Diabetes

Research Interests
We have two major research interests. The first one, our permanent interest, is to study tyrosine Phosphoprotein Signaling Networks that control cell mobility, proliferation, metabolism and cell transformation. Particularly, we are interested in studying how receptor tyrosine kinases like EGFR and Insulin Receptor interpret extracellular cues and integrate these signals into intracellular signaling modules and scaffolds that regulate the cellular dynamic processes. Mechanistic studies determine how they transmit signals that control the dynamic assemble of phosphorylated protein complexes to mediate the cell processes. We develop and utilize advanced phosphoproteomic approaches to discover novel tyrosine phosphorylated protein(s), linking(s) and cross-talk(s) in a proteome-scale. The long-term goal of the project is to identify novel pharmaceutical targets for diagnostic and treatment of cancer and diabetes. Cell Polarity has been implicated correlation with cell proliferation, migration as well as tumorigenesis. Thus, the second one, the newly emerged interest, is to understand how cell polarity protein complexes convert their polarity functions into other cellular processes in epithelia. The objective is to uncover novel molecular mechanisms that regulate cellular intrinsic linkage between the cell polarity and migration, proliferation, and tumorigenesis as well.

Selected Publications

  1. Du D, Xu F, Yu L, Zhang C, Lu X, Yuan H, Huang Q, Zhang F, Bao H, Jia L, Wu X, Zhu X, Zhang X, Zhang Z, Chen Z*. The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells. Development Cell. 2010, 18(1):52-63.
  2. Yuan H, Zhang H, Wu X, Zhang Z, Du D, Zhou S, Brakebusch C, and Chen Z*. Hepatocyte-specific deletion of Cdc42 results in delayed liver regeneration after partial hepatectomy in mice. Hepatology. 2009, 49:240-249.
  3. Lu XF, Feng XJ, Man XB, Yang G, Tang L, Du D, Zhang F, Yuan HX, Huang Q, Zhang Z, Liu YK, Strand D, and Chen Z*. Aberrant Splicing of Hugl-1, the Human Homologue of Drosophila Tumor Suppressor Gene lgl, is Associated with Hepatocellular Carcinoma Progression. Clin Cancer Res. 2009;15(10):3287-3296.
  4. Feng X, Lu X, Man X, Zhou W, Jiang LQ, Knyazev P, Lei L, Huang Q, Ullrich A, Zhang Z*, and Chen Z*. Overexpression of Csk binding protein (Cbp) contributes to renal cell carcinogenesis. Oncogene. 2009, 28(37):3320-31.
  5. Yang G, Li Q, Ren S, Lu X, Fang L, Zhou W, Zhang F, Xu F, Zhang Z, Zeng R, Lottspeich F, and Chen Z*. Proteomic, functional and motif-based analysis of C-terminal Src kinase-interacting proteins. Proteomics. 2009, 9(21):4944-61.
  6. Zhou W, Feng X, Wu Y, Benge J, Zhang Z, and Chen Z*. FGF-receptor substrate 2 functions as a molecular sensor integrating external regulatory signals into the FGF pathway. Cell Research. 2009, 19(10): 1165-1177.
  7. Ren S, Yang G, He Y, Wang Y, Li Y and Chen Z*. The conservation pattern of short linear motifs is highly correlated with the function of interacting protein domains. BMC Genomics. 2008, 9:452.
  8. Zhou Y, Fang L, Du D, Zhou W, Feng X, Chen J, Zhang Z, and Chen Z*. Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells. Acta Biochem Biochim Sin. 2008 Aug; 40(8):729-39.
  9. Fang L, Wang Y, Du D, Yang G, Kwok T, Kong S, Chen B, Chen D, Chen Z. Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair. Cell Research, 2007, 17(2):100-116
    (Highlights: A Polarity Protein Says Give Me a DNA Break. Cell, 2007, 128(3):419; The double (strand break) life of Par-3. Nature Cell Biology. 2007, 9(4):363-365.)
  10. Wang Y, Du D, Fang L, Zhang C, Yang G, Lottspeich F, Ullrich A, Chen Z. Tyrosine Phosphorylated Par3 Regulates Epithelial Tight Junction Assembly Promoted by EGFR Signaling. EMBO J. 2006, 5:5058-5070.
  11. Wang Y, Li R, Du D, Zhang C,Yuan H, Zeng R* and Chen Z*. Proteomic Analysis Reveals Novel Molecules Involved in Insulin Signaling Pathway. J Proteome Res. 2006, 5:846-855. (This paper is cited by JPR as a Featured Article)
  12. Lake Jiang Q, Feng X, Zhou W, Knyazev P, Ullrich A, Chen Z. Csk-binding protein(Cbp) negatively regulates epidermal growth factor-induced cell transformation by controlling Src activation. Oncogene. 2006, 25:5495-5506.
  13. Yuan H, Zhang L, Liu A, Zhou H, Wang Y, Zhang H, Wang G, Zeng R, Zhang Y*, Chen Z*. Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions. J. Proteome. Res. 2006, 5:299-307.
  14. Yao G, Chen W, Luo H, Jiang Q, Xia Z, Zang L, Zuo J, Wei X, Chen Z, Shen X, Dong C, Sun B. Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling. Int Immunol. 2006, 18(1):19-29.
  15. Zong X, Eckert C, Yuan H, Wahl-Schott C, Abicht H, Fang L, Li R, Mistrik P, Gerstner A, Much B, Baumann L, Michalakis S, Zeng R, Chen Z*, Biel M*. A Novel Mechanism of Modulation of Hyperpolarization-activated Cyclic Nucleotide-gated Channels by Src Kinase. J. Biol. Chem. 2005, 280(40): 34224-34232.
  16. Chen Z, Ullrich A. EGFR and FGFR signaling through FRS2 is subject to negative feedback control by ERK1/2. Biol. Chem. 2003, 384(8):1215-26.
  17. Chen Z*, Kharitonenkov A*, Sures I, Wang H, and Ullrich A. A Family of Proteins that Inhibit Signaling through Tyrosine Kinase Receptors. Nature, 1997, 386: 181-186.

Education Background & Academic Experience
Prof. Zhengjun Chen is employed by the "One Hundred Talents Program" of the Chinese Academy of Sciences. In March 2001, he returned from the Max-Plank Institute of Biochemistry in Germany to Shanghai Institute of Biochemistry and Cell Biology, and established the Group of Molecular Cancer Research. Mr. Chen has the background of medicine and was engaged in the signal transduction research before coming back to China. He will continue to research on cancer signal transduction, and explore the cancer proteome, especially the phospho-proteome realm, and Cancer signal transduction and proteomics.

Research Team

 

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