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Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
DING Jianping
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: jpding@@sibcb.ac.cn

Research Areas
X-ray crystallography; protein-protein interaction; structure-function relationship; mechanism; recognition; structure-based drug design; chromatin-remodeling proteins; histone modification; epigenetics; mTOR; metabolic enzymes; aminoacyl-tRNA synthetase; tryptophanyl-tRNA synthetase; antigen-antibody; protein complex.

Research Interests

Structural Biology of Regulation of Eukaryotic Gene Expression
Regulation of gene expression in eukaryotes is much more complex than in prokaryotes, and occurs at multiple levels or steps including replication, transcription, mRNA maturation and exportation, translation and post-translation. This complexity drives the processes of cellular differentiation and morphogenesis, leading to the creation of different cell types in multi-cellular organisms where different types of cells may possess different gene expression profiles although they all share the same genome materials. Integrity and stability of DNA are essential for living cells, and errors occurred in DNA replication and DNA damages caused by cellular metabolic processes or environmental factors have to be detected and repaired via various DNA repair pathways. Transcription is not only regulated by basal and regulatory transcription factors, but is also subjected to epigenetic regulation including covalent modifications of DNA, RNA and histones which can modulate the chromatin structure and subsequently activate or silence the transcription of specific genes. After transcription, mRNA must be processed, matured, and then exported to cytoplasm before it can be translated. Translation and post-translation can be regulated by various signaling pathways including the mTOR pathway and the endocytotic pathway. All of these processes involve numerous proteins and protein complexes with not well-understood functions and mechanisms. As the biological functions of proteins are largely dictated by their three-dimensional structures, a comprehensive structural and functional knowledge of the proteins will not only enhance the understanding of the structure-function relationship of macromolecules and the relevant biological processes, but also provide molecular basis for the discovery and development of new pharmaceuticals for diagnosis and treatment of human diseases.
Our group is interested in the studies of structures, functions, and molecular mechanisms of proteins and protein complexes involved in the regulation of gene expression in eukaryotes, in particular these of significant biological importance and/or related to human diseases. We utilize primarily structural biology in combination with biochemistry, molecular biology, cell biology, and other biological and biophysical methodologies to determine the three-dimensional structures of these proteins and their complexes with small ligands, substrates, and protein partners, and to elucidate the underlying molecular mechanisms of their biological functions. The primary goal of our research works is to understand the basic sciences of biology and in particular the structure-function relationships of proteins and protein complexes. The ultimate goal of our research works is to utilize the gained structural and functional knowledge to understand molecular basis of pathogenesis of human diseases and to develop effective drugs for the treatments of human diseases.
The current researches focus on the structural and functional studies of: (1) proteins involved in epigenetic regulation of eukaryotic genes, including histone acetyltransferases and deacetylases, histone methyltransferases and demethylases, and DNA£¯RNA methyltransferases and demethylases; (2) proteins involved in important signaling pathways, particularly the mTORC1 signaling and DNA damage repair pathways; and (3) important human enzymes involved in protein and nucleic acid metabolisms.

Selected Publications

Selected publications from 2012 to now (*Corresponding author) :

  1. Shicheng Zhang, Linlin Wang, Ye Tao, Tuya Bai, Rong Lu, Tianlong Zhang, Jiangye Chen, and Jianping Ding* ¡°Structural basis for the functional role of the Shu complex in homologous recombination¡±, Nucleic Acids Res., 45: 13068-13079 (2017).
  2. Tianlong Zhang$, Rong Wang$, Zhijing Wang, Xiangxiang Wang, Fang Wang, and Jianping Ding* ¡°Structural basis for Ragulator functioning as a scaffold in membrane-anchoring of Rag GTPases and mTORC1¡±, Nature Commun., 8: 1394 (2017). ($Contributed equally)
  3. Chen Zhong*, Jinlong Shen, Huibing Zhang, Guangyi Li, Senlin Shen, Fang Wang, Kuan Hu, Longxing Cao, Yongning He, and Jianping Ding* ¡°Cbln1 and Cbln4 are structurally similar but differ in GluD2 binding interactions¡±, Cell Rep., 20: 2328-2340 (2017).
  4. Ye Tao$, Chen Zhong$, Junjun Zhu, Shutong Xu, and Jianping Ding* ¡°Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2¡±, Nucleic Acids Res., 45: 5707-5719 (2017). ($Contributed equally)
  5. Tengfei Ma, Yingjie Peng, Wei Huang, Yabing Liu, and Jianping Ding* ¡°The and subunits play distinct functional roles in the a2bg heterotetramer of human NAD-dependent isocitrate dehydrogenase¡±, Sci. Rep., 7: 41882 (2017).
  6. Tengfei Ma, Yingjie Peng, Wei Huang, and Jianping Ding* ¡°Molecular mechanism of the allosteric regulation of the ag heterodimer of human NAD-dependent isocitrate dehydrogenase¡±, Sci. Rep., 7: 40921 (2017).
  7. Wenshuai Wang$, Xiaoyu Sun$, Yanbing Li$, Jinpeng Su$, Zhiyang Ling, Tianlong Zhang, Fang Wang, Hong Zhang, Hualan Chen*, Jianping Ding*, and Bing Sun* ¡°Human antibody 3E1 targets the HA stem region of H1N1 and H5N6 influenza A viruses¡±, Nature Comm., 7: 13577 (2016). ($Contributed equally)
  8. Jing Xia$, Rong Wang$, Tianlong Zhang*, and Jianping Ding* ¡°Structural insight into the arginine binding specificity of CASTOR1 in amino acid-dependent mTORC1 signaling¡±, Cell Disc., 2: 16035 (2016). ($Contributed equally)
  9. Rong Wang, Zhijing Wang, Tianlong Zhang*, and Jianping Ding* ¡°Structural basis for targeting BIG1 to Golgi apparatus through interaction of its DCB domain with Arl1¡±, J. Mol. Cell Biol., 8: 459-461 (2016).
  10. Fengjuan Lv, Tianlong Zhang, Zhen Zhou, Shuaixin Gao, Catherine CL Wong, Jin-Qiu Zhou, and Jianping Ding* ¡°Structural basis for Sfm1 functioning as a protein arginine methyltransferase¡±, Cell Disc., 1: 15037 (2015).
  11. Wenjing Li, Tianlong Zhang, and Jianping Ding* ¡°Molecular basis for the substrate specificity and catalytic mechanism of thymine-7-hydroxylase in fungi¡±, Nucleic Acids Res., 43: 10026-10038 (2015).
  12. Shaoqiong Xu$, Tianlong Zhang$, Shiva N. Kompella$, Mengdi Yan, Aiping Lu, Yanfang Wang, Xiaoxia Shao, Chengwu Chi, David J. Adams*, Jianping Ding*, and Chunguang Wang* ¡°Conotoxin aD-GeXXA utilizes a novel strategy to antagonize nicotinic acetylcholine receptors¡±, Sci. Rep., 5: 14261 (2015). ($Contributed equally)
  13. Qingyu Tang, Caifeng Liu, Chen Zhong, and Jianping Ding* ¡°Crystal structures of Arabidopsis thaliana Nudix hydrolase NUDT7 reveal a previously unobserved conformation¡±, Mol. Plant, 8: 1557-1559 (2015).
  14. Katie Powis$, Tianlong Zhang$, Nicolas Panchaud, Rong Wang, Claudio De Virgilio*, and Jianping Ding* ¡°Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling¡±, Cell Res., 25: 1043-1059 (2015). ($Contributed equally)
  15. Fang Yu$, Fangyuan He$, Hongyan Yao, Chengyuan Wang, Jianchuan Wang, Jianxu Li, Xiaofeng Qi, Hong-Wei Xue*, Jianping Ding*, and Peng Zhang* ¡°Structural basis of intramitochondrial phosphatidic acid transport mediated by the Ups1-Mdm35 complex¡±, EMBO Rep., 16: 813-823 (2015). ($Contributed equally)
  16. Lun Zhang, Jianchuan Wang, Li Hou, Pengrong Cao, Li Wu, Qiansen Zhang, Huaiyu Yang, Yi Zhang*, Jianping Ding* and Jia Li* ¡°Functional role of histidine in the conserved His-x-Asp motif in the catalytic core of protein kinases¡±, Sci. Rep., 5: 10115 (2015).
  17. Zhe Zhang$, Shanshan Wang$, Tong Shen, Jiangye Chen, and Jianping Ding* ¡°Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2¡±, Structure, 22, 1408-1420 (2014). ($Contributed equally)
  18. Zilong Zhang, Jian Wu, Wei Lin, Jin Wang, Han Yan, Wei Zhao, Jun Ma, Jianping Ding*, Peng Zhang*, and Guoping Zhao* ¡°Subdomain II of a-isopropylmalate synthase is essential for activity: inferring a mechanism of feedback inhibition¡±, J. Biol. Chem., 289, 27966-27978 (2014).
  19. Shicheng Zhang, Tianlong Zhang, Minghui Yan, Jianping Ding*, and Jiangye Chen* ¡°Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans¡±, Cell Res., 24, 1108-1120 (2014).
  20. Zhe Zhang, Tianlong Zhang, Shanshan Wang, Zou Gong, Chun Tang, Jiangye Chen, and Jianping Ding* ¡°Molecular mechanism for Rabex-5 GEF activation by Rabaptin-5¡±, eLife, 3, e02687 (2014).  
  21. Hui Yang, Tianlong Zhang, Ye Tao, Fang Wang, Liang Tong, and Jianping Ding* ¡°Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair¡±, Nucleic Acids Res., 41, 10573-10583 (2013).
  22. Shutong Xu, Wenjing Li, Junjun Zhu, Rong Wang, Zheng Li, Guo-Liang Xu, and Jianping Ding* ¡°Crystal structures of isoorotate decarboxylases reveal a novel catalytic mechanism of 5-carboxyl-uracil decarboxylation and shed lights on the search for DNA decarboxylase¡±, Cell Res., 23, 1296-1309 (2013).   
  23. Jianchuan Wang, Chen Zhong, Fang Wang, Fangfang Qu, and Jianping Ding* ¡°Crystal structures of S6K1 provide insights into the regulation mechanism of S6K1 by the hydrophobic motif¡±, Biochem. J., 454, 39-47 (2013).
  24. Ke Xu, Minhua Zhang, Qin Zhao, Fang Yu, Hui Guo, Chengyuan Wang, Fangyuan He, Jianping Ding, and Peng Zhang* ¡°Crystal structure of a folate ECF transporter from Lactobacillus brevis¡±, Nature, 497, 268-271 (2013).
  25. Wei Huang, Zhen Gong, Jian Li, and Jianping Ding* ¡°Crystal structure of Drosophila melanogaster tryptophan 2,3-dioxygenase reveals insights into substrate recognition and catalytic mechanism¡±, J. Struct. Biol., 181, 291-299 (2013).
  26. Sisi Li, Jiamu Du, Hui Yang, Juan Yin, Jianping Ding*, and Jiang Zhong* ¡°Functional and structural characterization of DNMT2 from Spodoptera frugiperda¡±, J. Mol. Cell Biol., 5, 64-66 (2013).
  27. Kuan Hu, Meng Zhao, Tianlong Zhang, Manwu Zha, Chen Zhong, Yu Jiang, and Jianping Ding* ¡°Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutulicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism¡±, Biochem. J., 449, 79-89 (2013).
  28. Wenjing Li, Chen Zhong, Lei Li, Bingfa Sun, Weiyi Wang, Shutong Xu, Tianlong Zhang, Chunguang Wang, Lan Bao, and Jianping Ding* ¡°Molecular basis of the acetyltransferase activity of MEC-17 towards ¦Á-tubulin¡±, Cell Res., 22, 1707-1711 (2012).
  29. Tianlong Zhang, Marie-Pierre P¨¦li-Gulli, Hui Yang, Claudio De Virgilio, and Jianping Ding* ¡°Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the EGO complex to activate TORC1¡±, Structure, 20, 2151-2160 (2012).
  30. Manwu Zha, Chen Zhong, Ying Ou, Li Han, Jianchuan Wang, and Jianping Ding* ¡°Crystal structures of human CaMKIa reveal insights into the regulation mechanism of CaMKI¡±, PLoS One, e44828 (2012).
  31. Hui Yang, Tianlong Zhang, Ye Tao, Lijing Wu, Hong-tao Li, Jin-qiu Zhou, Chen Zhong, and Jianping Ding* ¡°Saccharomyces cerevesiae MHF complex structurally resembles the histones (H3-H4)2 heterotetramer and functions as a heterotetramer¡±, Structure, 20, 364-370 (2012).

Education Background & Academic Experience
Jianping Ding, Ph.D. B.S., Nanjing University, 1982; M.S., Zhongshan University, 1984; Ph.D., Fudan University, 1987. 11/1987-6/1989, Postdoctoral Associate, Shanghai Institute of Ceramics, Chinese Academy of Sciences; 7/1989-8/1991, Research Fellow of the Alexander von Humboldt Foundation, Institute for Crystallography, Free University of Berlin, Germany; 9/1991-1/2001, Assistant/Associate Research Professor, Rutgers University Department of Chemistry, and Senior Research Scientist, Center for Advanced Biotechnology and Medicine, Rutgers University, USA; 11/2000-Present, Professor, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences.

Research Team


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