Home CAS Center for Excellence


Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: zr@@sibcb.ac.cn

Research Areas
proteomics;mass spectrometry;high-throughput protein identification; protein-protein interaction network;protein dynamics; quantitiative proteomics;subcelluar proteomics;adipocyte differentiation; diabetes

Research Interests
       1. Methodology development in Proteomics: Develop mass spectrometry based methods including multi-dimensional LC-MS/MS for protein profiling in cellular and subcellular levels, phosphopeptide enrichment and multiplex quantitation. Apply novel strategies to investigate the protein dynamic expression, location, modification and interaction in diverse model systems.
        2. Application of quantitative proteomics in cell signaling: Monitor protein dynamic phosphorylation and find out new interactions and components in Wnt signaling pathways and adipocyte differentiation.
        3. Application of quantitative proteomics in biomarker discovery of diabetes: Analyze the tissue and serum proteomes to track the progression of diabetes in human and animal models.
         4. Investigation of regulation mechanism of protein dynamic behaviors: Combine Proteomic data with epigenetic, transcriptomic and MiRNA data etc., to reveal system-wide association of biological molecules.

Selected Publications

  1. Wang Q, Zhang Y, Yang C, Xiong H, Lin Y, Yao J, Li H, Xie L, Zhao W, Yao Y, Ning ZB, Zeng R, Xiong Y, Guan KL, Zhao S, Zhao GP. Acetylation of metabolic enzymes coordinates carbon source utilization and metabolic flux. Science. 2010, 327:1004-7.
  2. Deng WJ, Nie S, Dai J, Wu JR, Zeng R*. Proteome, phosphoproteome and hydroxyproteome of liver mitochondria in diabetic rats at early pathogenic stages. Mol Cell Proteomics. 2010, 9:100-16.
  3. Wu YB, Dai J, Yang XL, Li SJ, Zhao SL, Tang JS, Zheng GY, Li YX, Wu JR, Zeng R*. Concurrent quantification of proteome and phosphoproteome to reveal system-wide association of protein phosphorylation and gene expression. Mol Cell Proteomics. 2009, 8:2809-26.
  4. Li H, Xing X, Ding G, Li Q,Wang C, Xie L, Zeng R*, Li Y. SysPTM - a systematic resource for proteomic research of post-translational modifications. Mol Cell Proteomics. 2009. 8:1839-49.
  5. Dai J, Wang LS, Wu YB, Sheng QH, Wu JR, Shieh CH, Zeng R*. Fully Automatic Separation and Identification of Phosphopeptides by Continuous pH-Gradient Anion Exchange Online Coupled with Reversed-Phase Liquid Chromatography Mass Spectrometry. J Proteome Res. 2009, 8:133-41.
  6. Li SJ, Peng M, Li H, Liu BS, Wang C, Wu JR, Li YX, Zeng R*. Sys-Body Fluid: a systematical database for human body fluid proteome research. Nucl. Acids Res. 2009, 37:D907-12.
  7. Ning ZB, Li QR, Dai J, Li RX, Shieh CH, Zeng R*. Fractionation of complex protein mixture by virtual three-dimensional liquid chromatography based on combined pH and salt steps. J Proteome Res. 2008, 7:4525-37.
  8. Dai J, Jin WH, Sheng QH, Shieh CH, Wu JR, Zeng R*. Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry. J Proteome Res. 2007, 6: 250-62.
  9. Sheng QH, Wang LS, Dai J, Jiang XS, Li RX, Ma DJ, Li YX, Zeng R*, Wu JR. Comparison of a proteomic approach with a microarray-based approach to detect exons in the mouse genome. Nat Genet. 2006, 38:1223-4.
  10. Dai J, Shieh CH, Sheng QH, Zhou H, Zeng R*. Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps. Anal Chem. 2005, 77:5793-9.
  11. Tu CJ, Dai J, Li SJ, Sheng QH, Deng WJ, Xia QC, Zeng R*. High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification. J Proteome Res. 2005, 4:1265-73.
  12. Li Z, Dong X, Wang Z, Liu W, Deng N, Ding Y, Tang L, Hla T, Zeng R, Li L, Wu D. Regulation of PTEN by Rho small GTPases. Nat Cell Biol. 2005, 7:399-404.
  13. Li C, Tan YX, Zhou H, Ding SJ, Li SJ, Ma DJ, Man XB, Hong Y, Zhang L, Li L, Xia QC, Wu JR, Wang HY, Zeng R*. Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers. Proteomics. 2005, 5:1125-39.
  14. Jiang XS, Tang LY, Dai J, Zhou H, Li SJ, Xia QC, Wu JR, Zeng R*. Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection. Mol Cell Proteomics. 2005, 4:902-13.
  15. Jiang XS, Dai J, Sheng QH, Zhang L, Xia QC, Wu JR, Zeng R*. A comparative proteomic strategy for subcellular proteome research: ICAT approach coupled with bioinformatics prediction to ascertain rat liver mitochondrial proteins and indication of mitochondrial localization for catalase. Mol Cell Proteomics. 2005, 4:12-34.

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