Home CAS Center for Excellence


Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
ZHU Xueliang
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: xlzhu@@sibcb.ac.cn

Research Areas
mitosis; cell migration; kinetochore; motor proteins; mitosin; Nude/Nudel; Lis1; dynein; Cdc42GAP

Research Interests
        My group is mainly interested in regulation of cell motilities, including mitosis, migration, and intracellular transport. Such activities often exhibit transient, regional distributions of organelles and/or proteins, require accurate coordination among multiple participants, and involve MT and microfilament cytoskeletons.
        We have recently focused on a genetic pathway, the dynein pathway. It was originally identified in Aspergillus in screens for mutants critical for even nuclear distrubutions (Nud) along hyphae and subsequently found to be conserved and essential in mammals. Whereas the majority of Nud genes identified encode subunits of cytoplasmic dynein and dynactin, two genes termed NudE and NudF, corresponding to NudE/NudE-like (Nudel) and Lis1 in mammals, respectively, are of interest because they potentially code for dynein regulators. Moreover, halploinsufficiency of Lis1 is known to cause a severe congenital disease, type I lissencephaly, characterized by smooth brain surface, mental retardation, and short life span. Dynein and dynactin consist of a huge microtubule-dependent molecular motor important for many types of cell motilities. Malfunction of the dynein motor has been shown to cause Amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disease that affects neurons in the brain and the spinal chord.
        Starting from mitosin/CENP-F, an Rb-associated kinetochore protein, we identified NudE and Nudel as its associated proteins. Since then, we have tried to answer how they function in cell motilities in respect to other related proteins.

Selected Publications

  1. Li Y, Guo F, Jing Q, Zhu X*, Yan X*(2020). Characterization of centriole biogenesis during multiciliation in planarians. Biol Cell doi: 10.1111/boc.202000045.
  2. Zhao H, Yang S#, Chen Q, Duan X, Li G, Huang Q, Zhu X*, Yan X*(2020). Cep57 and Cep57l1 function redundantly to recruit the Cep63CCep152 complex for centriole biogenesis J Cell Sci 133(13):jcs241836.
  3. Chen Y, Zheng J#, Li X., Zhu L, Shao Z, Yan X*, and Zhu X*(2020). Wdr47 controls neuronal polarization through the Camsap family microtubule minus-end-binding proteins. Cell Rep 31(3):107526.
  4. Chen Y, Zheng J, Li X., Zhu L, Shao Z, Yan X*, and Zhu X*(2020). Wdr47 controls neuronal polarization through the Camsap family microtubule minus-end-binding proteins. Cell Rep. Doi:10.1016/j.celrep.2020.107526.
  5. Xiao Y, Chen J, Wan Y, Gao Q, Jing N, Zheng Y and Zhu X* (2019). Regulation of zebrafish dorsoventral patterning by phase separation of RNA-binding protein Rbm14. Cell Discov 5:37.
  6. Zhao H, Chen Q, Fang C, Huang Q, Zhou J, Yan X* and Zhu X* (2019). Parental centrioles are dispensable for deuterosome formation and function during basal body amplification. EMBO Reports 20(4). pii: e46735.
  7. Zheng J, Liu H, Zhu L, Chen Y, Zhao H, Zhang W, Li F, Xie L, Yan X* and Zhu X*(2019). Microtubule-bundling protein Spef1 enables mammalian ciliary central apparatus formation. J Mol Cell Biol 11(1):67-77.
  8. Zhang Y,  Chen Y, Zheng J, Wang J,  Duan S, Zhang W, Yan X* and Zhu X* (2019). Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. J Mol Cell Biol 11(5):383-394.
  9. Li G, Huang S, Yang S, Wang J, Cao J, Czajkowsky D, Shao Z, and Zhu X* (2018). abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing. Cell Discov 4:42. 
  10. Zhu L, Liu H, Chen Y, Yan X*, and Zhu X* (2018). Rsph9 is critical for ciliary radial spoke assembly and central pair microtubule stability. Biol Cell 111(2): 29-38. 
  11. Yu F, Guo S, Li T, Ran J, Zhao W, Li D, Liu M, Yan X, Yang X, Zhu X, and Zhou J* (2018). Ciliary defects caused by dysregulation of O-GlcNAc modification are associated with diabetic complications. Cell Res 29(2):171-173.
  12. Huang Y, Li T#, Ems‑McClung S, Walczak C, Prigent C, Zhu X, Zhang X, and Zheng Y* (2018).Aurora A activation in mitosis promoted by BuGZ. J Cell Biol 217(1):107-116.
  13. Yan X, Zhao H and Zhu X*(2016). Production of Basal Bodies in bulk for dense multicilia formation. F1000 Research 5(F1000 Faculty Rev):1533 (doi: 10.12688/f1000research.8469.1). Review
  14. Yan X and Zhu X* (2016).PP2A in meiotic oocytes. Cell Cycle 15(15):1950-1951. Review
  15. Zeng L, Cai C, Li S, Wang W, Li Y, Chen J, Zhu X*, Zeng YA* (2016). Essential Roles of Cyclin Y-Like 1 and Cyclin Y in Dividing Wnt-Responsive Mammary Stem/Progenitor Cells. PLoS Genet. 12(5): e1006055.
  16. Jiang H, He X, Feng D, Zhu X*, Zheng Y*(2015). RanGTP aids anaphase entry through Ubr5-mediated protein turnover. J Cell Biol. 211(1):7-18.
  17. Jiang H, Wang S, Huang Y, He X, Cui Hong, Zhu X*, and Zheng Y* (2015). Phase transitions of spindle-associated protein regulate spindle apparatus assembly Cell. 163(1):108-22.
  18. Wan Y, Zheng X, Chen H, Guo Y, Jiang H, He X, Zhu X*, and Zheng Y*(2015). Splicing function of mitotic regulators links R-loopCmediated DNA damage to tumor cell killing. J Cell Biol. 209(2), 235-46.
  19. Xu Y, Cao L, Huang S, Feng D, Zhang W, Zhu X* and Yan X* (2015). Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PLoS ONE. 10(4):e0124378.
  20. Jiang H, He X, Wang S, Jia J, Wan Y, Wang Y, Zeng R, Yates III J, Zhu X*, and Zheng Y* (2014). A microtubule-associated zinc finger protein, BuGZ, regulates mitotic chromosome alignment by ensuring Bub3 stability and kinetochore targeting. Dev Cell. 28(3):268-281.
  21. Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X* and Zhu X*(2013). The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. Nat Cell Biol. 15(12):1434-44.
  22. Zeng L, Wan Y, Li D, Wu J, Shao M, Chen J, Hui L, Ji H,and Zhu X* (2013). The m subunit of murine translation initiation factor eIF3 maintains the integrity of the eIF3 complex and is required for embryonic development, homeostasis, and organ size control. J Biol Chem. 288(42):30087-93.
  23. Zhou Y, Li S, Huang Q, Xie L, Zhu X* (2013). Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3. J Mol Cell Biol. 5(4):239-49.
  24. Yan X and Zhu X* (2013). Branched F-actin as a negative regulator of cilia formation. Exp Cell Res. 319(2):147-151
  25. Cao J, Shen Y, Zhu L, Xu Y, Zhou Y, Wu Z, Li Y, Yan X* and Zhu X* (2012). miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Nat Cell Biol. 14:697-706.
  26. Wan Y, Yang Z, Guo J, Zhang Q, Zeng L, Song W, Xiao Y, and Zhu X* (2012). Misfolded G beta is recruited to cytoplasmic dynein by Nudel for efficient clearance. Cell Res. 22: 1140-1154
  27. Wu S, Ma L, Wu Y, Zeng R, and Zhu X* (2012). Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions. Cell Res. 22:1270-1284
  28. Zhao H, Zhu L, Jin Y, Ji H, Yan X* and Zhu X* (2012). miR-375 is highly expressed and possibly transactivated by ASCL1 in small cell lung cancer cells. Acta Biochim Biophys Sin. 44(2):177-82.
  29. Du J, Li Y, Zhu X* (2010). Involvement of CENP-F in histone methylation. Acta Biochim Biophys Sin. 42(3):173-6.
  30. Du J, Zhang Y, Liu Y, Li Y, and Zhu X* (2010). Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase. Acta Biochim Biophys Sin. 42: 839C846.
  31. Yan X, Shen Y, Zhu X* (2010). Live Show of Rho GTPases in Cell Migration. J Mol Cell Biol. 2: 68C69.
  32. Zhu X* (2010). Seeing the Yin and Yang in Cell Biology. Mol Biol Cell. 21: 3827C3828.
  33. Ding C, Liang X, Ma L, Yuan X and Zhu X*. (2009) Opposing effects of Ndel1 and alpha1 or alpha 2 on cytoplasmic dynein through competitive binding to Lis1. J Cell Sci. 122, 2820-2827.
  34. Zhang Q, Wang F, Cao J, Shen Y, Huang Q, Bao L and Zhu X*. (2009) Nudel promotes axonal lysosome clearance and endo-lysosome formation via dynein-mediated transport. Traffic. 10:1337-1349.
  35. Shan Y, Yu L, Li Y, Pan Y, Zhang Q, Wang F, Chen J, and Zhu X* (2009). Nudel and FAK as antagonizing strength modulators of nascent adhesions through Paxillin. PLoS Biol. 7(5): e1000116.
  36. Ma L, Tsai M-Y, Wang S, Lu B, Chen R, Yates III JR, Zhu X*, and Zheng Y* (2009). Requirement for Nudel and dynein for assembly of the lamin B spindle matrix. Nat Cell Biol. 11: 247-256.
  37. Shen Y, Li N, Wu S, Zhou Y, Shan Y, Zhang Q, Ding C, Yuan Q, Zhao F, Zeng R, Zhu X*. (2008) Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells. Dev Cell. 14, 342-353.
  38. Wang F, Zhang Q, Cao J, Huang Q, Zhu X*. (2008) The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules. Exp Cell Res. 314, 213-226.
  39. Li Y, Yu W, Liang Y, Zhu X*. (2007) Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment. Cell Res. 17, 701-712.
  40. Liang Y, Yu W, Li Y, Yu L, Zhang Q, Wang F, Yang Z, Du J, Huang Q, Yao X, and Zhu X*. (2007) Nudel modulates kinetochore association and function of cytoplasmic dynein in M phase. Mol Biol Cell. 18, 2656-2666.
  41. Guo J, Yang Z, Song W, Chen Q, Wang F, Zhang Q, and Zhu X*. (2006) Nudel contributes to MT anchoring at the mother centriole and is involved in both dynein-dependent and independent centrosomal protein assembly. Mol Biol Cell. 17:680-9.
  42. Yang Z, Guo J, Chen Q, Du J, Ding C, and Zhu X*. (2005) Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death. Mol Cell Biol. 25, 4062-74.
  43. Zhou X, Wang R, Fan L, Li Y, Ma L, Yang Z, Yu W, Jing N, and Zhu X*. (2005) Mitosin/CENP-F as a negative regulator of activating transcription factor-4. J Biol Chem . 280, 13973-7.
  44. Liang Y, Yu W, Li Y, Yang Z, Yan X, Huang Q, and Zhu X*. (2004) Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J Cell Biol. 164, 557-566.
  45. Yang Z, Guo J, Li N, Qian M, Wang S, Zhu X*. (2003) Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport. Cell Res. 13, 275-283.
  46. Fan L, Yu W, Zhu X*. (2003) Interaction of Sedlin with chloride intracellular channel proteins. FEBS Lett. 540, 77-80.
  47. Yan X, Li F, Liang Y, Shen Y, Zhao X, Huang Q, and Zhu X*. (2003) Human Nudel and NudE as Regulators of Cytoplasmic Dynein in Poleward Protein Transport along the Mitotic Spindle. Mol Cell Biol. 23, 1239-50.
  48. Zhao X, Yang Z, Qian M, and Zhu X*. (2001) Interaction among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochem Biophy Res Commun. 280, 513-517.
  49. Yan X., Zhao X, Qian M, Guo N, Gong X, and Zhu X*. (2000) Characterization and gene structure of a novel retinoblastoma-protein-associated protein similar to the transcription regulator TFII-I. Biochem J. 345, 749-757.
  50. Zhu X*. (1999) Structural requirements and dynamics of mitosin-kinetochore interaction in M phase. Mol Cell Biol. 19, 1016-1024.
  51. Zhu X*, Ding L, and Pei G (1997). Carboxyl terminus of mitosin is sufficient to confer spindle pole localization. J Cell Biochem. 66:441-449.

Education Background & Academic Experience
Xueliang Zhu, B. Sc., M. Sc. (University of Science and Technology of China, USTC), Ph.D. (Shanghai Institute of Cell Biology, the Chinese Academy of Sciences). Associate professor since 1997. Professor since 1999.

Research Team


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