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Faculty

Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
 
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
 
Principal Investigators
 
LIAO Kan
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: kliao@@sibs.ac.cn

Research Areas
adipocyte differentiation; cell proliferation; IGF-1 receptor signaling; caveolae; lipid rafts; centrosome; protein kinase B/Akt; cortactin; GLUT4; primary cilium; foam cell; macrophage; atherosclerosis; protein-lipid interaction; F-actin

Research Interests
Cell differentiation and proliferation are two reciprocally inhibited events. Growth arrest is the first step for 3T3-L1 adipocyte differentiation (1-3). Adipocyte differentiation can not be induced in proliferating 3T3-L1 cells. To induce adipocyte differentiation, growth arrested 3T3-L1 preadipocyte is induced by hormonal regimen (isobutylmethylxanthine, dexamethasone and insulin) (4-7). IGF-1 receptor signal is one of the most important induction signals (6-8). We are interested in how growth arrest prepares 3T3-L1 preadipocyte for differentiation induction and its effect on IGF-1 receptor signal transduction. We are also interested in the function of membrane lipid microdomains in IGF-1 receptor transmembrane signaling. 
Protein tyrosine phosphorylation and mitotic regulation are often related to each other. The identification of tyrosine phosphorylated cortactin in regulation of centrosome separation has prompted us to further explore the function of cortactin and its phosphorylation. As an anchor for F-actin, tyrosine phosphorylated cortactin provides a regulation of F-actin attachment by phosphorylation turnover. By regulating cortactin tyrosine phosphorylation, F-actin driven cellular movement can be directed and controlled (9). We are especially interested in the proteins interacted with tyrosine phosphorylated form of cortactin and their functions in microfilament system.

Selected Publications

  1. Wang W, Chen L, Ding Y, Jin J, Liao K. Centrosome separation driven by actin-microfilaments during mitosis is mediated by centrosome-associated tyrosine-phosphorylated cortactin. J Cell Sci. 2008 Apr 15;121(Pt 8):1334-43.
  2. Yuan T, Hong S, Yao Y, Liao K. Glut-4 is translocated to both caveolae and non-caveolar lipid rafts, but is partially internalized through caveolae in insulin-stimulated adipocytes. Cell Res. 2007 Sep;17(9):772-82.
  3. Xu J, Liao K. Protein kinase B/AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiation. J Biol Chem. 2004 Aug 20;279(34):35914-22.
  4. Hong S, Huo H, Xu J, Liao K. Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae. Cell Death Differ. 2004 Jul;11(7):714-23.
  5. Hairong Huo, Xuemin Guo, Shangyu Hong, Manrong Jiang, Xinyuan Liu, Kan Liao. Lipid Rafts/Caveolae Are Essential for Insulin-like Growth Factor-1Receptor Signaling during 3T3-L1 Preadipocyte Differentiation Induction. J Biol Chem, 2003 Mar 28;278(13):11561-9
  6. Zilong Qiu, Yong Wei, Nan Chen, Manrong Jiang, Jiarui Wu, Kan Liao. DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocytes differentiation into adipocytes. J Biol Chem. 2001;275:11988-11995.
  7. Bo Zhai, Hairong Huo, Kan Liao. C3G, a Guanine nucleotide exchange factor bound to adapter molecule c-Crk, has two alternative splicing forms. Biochem Biophy Res Comm, 2001;286:61-66.
  8. Qingwei Zhu, Jiang Zhu, Kan Liao. A Sp1-like cis-element is the major DNA motif for differential expression regulation of the adipocyte amino acid transporter. Biochem Biophy Res Comm, 2000;271:91-99.
  9. Qingwei Zhu, Kan Liao. Differential expression of the adipocyte amino acid transporter is transactivated by Sp1 and Sp3 during the 3T3-L1 preadipocyte differentiation process. Biochem Biophy Res Comm, 2000;271:100-106.
  10. Xuemin Guo, Kan Liao. Analysis of gene expression profile during 3T3-L1 preadipocyte differentiation. Gene, 2000;251:45-53.
  11. Shenghao Jin, Bo Zhai, Zilong Qiu, Jiarui Wu, M. Daniel Lane, Kan Liao. c-Crk, a substrate of the insulin-like growth factor receptor tyrosine kinase, functions as an early signal mediator in the adipocyte differentiation process. J Biol Chem. 2000;275:34344-34352.

Education Background & Academic Experience
Dr. Kan Liao, was born in Oct. 1962. Graduated from University of Science and Technology of China and obtained a Bachelor degree in 1984. In 1991, received his Ph.D. degree from the Johns Hopkins University, School of Medicine. From 1991 to 1994 worked at Johns Hopkins medical school as postdoctoral fellow. From 1994 to 1995 was a faculty member at University of Science and Technology of China. From 1995 to 1999 was a group leader in Shanghai Research Center for Life Sciences. Since 1999, he has been a group leader in Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science.

Research Team

 

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