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Members of CAS, CAE
National Outstanding Young Scientists Award
Principal Investigators
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
GAO Dong
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Email: dong.gao@@sibcb.ac.cn

Research Areas
Cell Plasticity and Cancer Therapy

Research Interests
The tumor response to most therapeutic agents in cancer is highly unpredictable. Cancer models which can adequately represent tumor heterogeneity and predict in vivo drug sensitivity are intense areas of investigation. Recently, cancer ^organoid ̄ culture conditions have been developed to establish in vitro growth of patient-derived samples at higher efficiency and they are very promising for large scale drug screening and fundamental cancer biology research.
In this laboratory, we combine organoid culture system, bioinformatic analysis and genetic engineered mouse models to define and characterize prostate luminal progenitor cells and prostate cancer progenitor cells, to develop new biomarker of advanced prostate cancers, to establish in vitro cancer organoids at different stage, to study drug resistant with tumor microenvironment and tumor cells plasticity, to establish Chinese patients derived cancer organoid bank.

Selected Publications

  1. Gao D and Chen Y. Organoid Development in Cancer Genome Discovery. Current Opinion in Genetics and Development. 2015 Mar 18; 30:42-48. (Invited Cover Review).
  2. Gao D*, Vela I*, Sboner A*, Iaquinta PJ*, Karthaus WL, Gopalan A, Wanjala JN, Dowling C, Undvall EA, Wongvipat J, Kossai M, Barboza LP, Di W, Cao Z, Zhang QF, Sirota I, Ran Ll, Solomon SB, MacDonald TY, Beltran H, Mosquera J, Eastham JA, Touijer KA, Scardino PT, Laudone VP, Rathkopf DE, Morris MJ, Danila DC, Slovin SF, Chi P, Clevers H, Carver B, Rubin MA, Scher HI, Sawyers CL, Chen Y. Generation of in vitro organoids cultures derived from patients with advanced prostate cancer. Cell, 2014 Sep; 159: 176-187. * co-first author
  3. Drost J*, Karthaus WR*, Gao D, Driehuis E, Sawyers CL, Chen Y and Clevers H. Organoid culture systems for prostate epithelial tissue and prostate cancer tissue. Nature Protocols. 2016 Jan; Doi:10.1038/347C358. * co-first author
  4. Wiesner T, Lee W, Obenauf A, Ran L, Murali R, Zhang Q, Wong E, Hu W, Sasinya S, Shah R, Scott S, Sboner A, Button J, Landa I, Gao D, Cao Z, Shukla S, Merghoub T, Schwartz G, Wang L, Ladanyi M, Fagin J, Hollmann T, Busam K, BergerM , Chen Y and Chi P. Alternative transcription initiation leads to expression of a novel ALK isoform in cancer. Nature. 2015 Oct; Doi:10.103811
  5. Ran LL, Sirota I, Cao Z, Murphy D, Chen YD, Shukla S, Xie YY, Gao D, Zhu S, Rossi F, Wongvipat J, Taguchi T, Tap WD, Mellinghoff IK, Besmer P, Antonescu CR, Chen Y and Chi P. Combined inhibition of MAP kinase and KIT signaling pathways destabilizes the ETV1 protein and synergistically suppress GIST tumorigenesis. Cancer Discovery. 2015 Jan; DOI: 10.1158/2159-829011
  6. Karthaus WR, Iaquinta PJ, Drost J, Gracanin A, Wongvipat J, Gao D, Befthel H, Sachs N, Vries RG.J, Cuppen E, Chen Y, Sawyers CL and Clevers H. Identification of luminal stem cells in the human prostate using a novel epithelial culture system. Cell, 2014 Sep; 159: 163-175.
  7. Zeng Y#, Gao D, Kim JJ, Shiraishi T, Terada N, Kakehi Y, Kong CZ, Getzenberg RH, Kulkarni P#. Prostate-associated gene 4 (PAGE4) protects cells against stress by elevating p21 and suppressing reactive oxygen species production. American Journal of Clinical and Experimental Urology, 2013 Dec; 1(1):39-52.
  8. Chen Y, Chi P, Rockowitz S, Iaquinta P.J, Shamu T, Shukla S, Gao D, Sirota I, Carver B.S, Wongvipat J, Scher H.I, Zheng D, Sawyers C.L. ETS factors reprogram the androgen receptor cistrome and prime prostate tumorigenesis in response to PTEN loss. Nature Medicine, 2013 Aug; 19(8):1023-9.
  9. Zeng Y#, Wodzenski D, Gao D, Shiraishi T, Terada N, Li Y, Griend D.J.V, Luo J, Kong C, Getzenberg R.H, Kulkarni P#. Stress-response protein RBM3 attenuates the stem-like properties of prostate cancer cells by interfering with CD44 variant splicing. Cancer Research. 2013 Jul; 73(13): 4123-4133.
  10. Yang YK, Qu H, Gao D, Zhai ZH, and Chen DY. ARF-like protein 16(ARL16) inhibits RIG-I by binding with CTD in GTP dependent manner. J Biol Chem. 2011, 286: 10568-10580.
  11. Gao D, Yang YK, Wang RP, Zhou X, Diao FC, Li MD, Zhai ZH, Jiang ZF, and Chen DY. REUL is a novel E3 ubiquitin ligase and stimulator of retinoic-acid-inducible gene-I. PLoS ONE. 2009; 4(6):e5760.
  12. Gao D*, Wang RP*, Li B, Yang Y, Zhai ZH, and Chen DY. WDR34 is a novel TAK1-associated suppressor of the IL-1R/TLR3/TLR4-induced NF-kappaB activation pathway. Cell Mol Life Sci. 2009; 6615:2573-84. * co-first author
  13. Zhang M, Tian Y, Wang RP, Gao D, Zhang Y, Diao FC, Chen DY, Zhai ZH, and Shu HB. Negative feedback regulation of cellular antiviral signaling by RBCK1-mediated degradation of IRF3. Cell Research. 2008 Nov; 18(11):1096-104.
  14. Zhang M, Wang RP, Diao FC, Lu F, Gao D, Chen D, Zhai ZH, and Shu HB. A CXXC ring finger protein is required for DNA damage-induced p53 activation. Science in China Series C: Life Science. 2009; 52(6):528-538.

Education Background & Academic Experience
Education Background & Academic Experience
2016-Present: Principal Investigator, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
2012-2016: Research Fellow, Human Oncology and Pathogenesis Program; Memorial Sloan Kettering Cancer Center, NY, U.S.A.
2011-2012: Postdoctoral Fellow, The James Buchanan Brady Urological Institute; The Johns Hopkins University, MD, U.S.A.
2006-2011: Ph.D., School of Life Science, Peking University, Beijing, China.
2001-2005: B.S., School of Life Science, Northeast Forest University, Haerbin, China.

2015: NYSTEM Postdoctoral Fellowship
2011: "Outstanding Graduates Awards" from Peking University
2011: Proteintech Scholarship
2009: Dong Gang Scholarship
2008: Zhang Jingyue Scholarship


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