 |
CHEN Lingling
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Tel: Email: linglingchen@sibcb.ac.cn |
Research Interests
The complete sequence of the human genome provided quite a surprise to many by revealing that more than 98% of the transcriptional output represents non-protein coding RNAs (ncRNAs), which include the classical "housekeeping" ncRNAs (tRNAs, rRNAs, and snRNAs). These include small/short ncRNAs (miRNAs and piRNAs) and long ncRNAs (lncRNAs). While the "housekeeping" ncRNAs and regulatory small/short ncRNAs are now universally acknowledged, long non-coding RNAs (lncRNAs, >200 nt) have only recently emerged as a major class of eukaryotic transcripts.
We study the regulatory function of lncRNAs in mammalian cells. Rather than accumulating silently in the cell, lncRNAs have been recently implicated in nuclear architecture, epigenetics and the regulation of gene expression. We are particularly interested in lncRNAs that are involved in nuclear architecture and the renewal of human embryonic stem cells (hESCs). Using state-of-the-art sequencing technology, we have recently finished the deep sequencing of several undifferentiated hESC and carcinoma cell lines and identified a number of novel lncRNAs that are specifically transcribed in hESCs. In our laboratory, we will exploit a combination of molecular biological, cell biological, and biochemical approaches to characterize these lncRNAs and to understand their novel roles in the renewal of hESCs.
These studies have the potential to not only significantly advance our understanding of the mechanisms and functional roles of lncRNA regulation in the mammalian genome, but also to provide pioneering insights into the regulation of stem cell pluripotency and self-renewal by these transcripts.
http://www.frontiersin.org/genetics
Selected Publications
-
Yin QF, Yang L, Zhang Y, Xiang JF, Wu YW, Carmichael GG and Chen LL. (2012) Long noncoding RNAs with snoRNA ends. Mol Cell. In press.
-
Chen LL and Carmichael GG. Nuclear editing of mRNA 3กฏ-UTRs. (2012) Curr. Top. Microbiol. Immunol. 353:111-121
-
Yang L, Duff MO, Graveley BR, Carmichael GG and Chen LL. Genomewide characterization of non-polyadenylated RNAs. (2011) Genome Biol. 12: R16.
-
Peng S, Chen LL, Chen F, Lei XX, Yang L, Qiu C, Lin H, Carmichael GG, Huang Y. Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells. (2011) Stem Cells. 29: 496-504.
-
Chen LL, Yang L and Carmichael GG. Molecular basis for an attenuated cytoplasmic dsRNA response in human embryonic stem cells. (2010) Cell Cycle. 9:3552-3564 (Commentary: Williams BRG (2010) Cell Cycle. 9:3395-3400.)
-
Chen LL and Carmichael GG. Long non-coding RNAs in mammalian cells: What, Where and Why? (2010) WIREs RNA. 1: 2-21.
-
Chen LL and Carmichael GG. Decoding the function of nuclear long non-coding RNAs. (2010) Curr. Opin. Cell Biol. 22: 357-364.
-
Chen LL and Carmichael GG. Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA. (2009) Mol Cell. 35: 467-478 (Commentaries by: Scadden D. (2009) Mol Cell. 35: 395-396; Faculty of 1000 Biology.)
-
Chen LL and Carmichael GG. Gene regulation by SINES and inosines: biological consequences of A-to-I editing of Alu element inverted repeats. (2008) Cell Cycle. 7: 3297-3301.
-
Chen LL, Decerbo JN and Carmichael GG. Alu element-mediated gene silencing. (2008) EMBO J. 27: 1694-1705.
-
Zhou J, Wang Q, Chen LL and Carmichael GG. On the mechanism of induction of heterochromatin by the RNA-binding protein vigilin. (2008) RNA 14: 1773-1781.
-
Zhou J, Chen LL and Carmichael GG. A role for A to I editing in gene silencing. (2008) in "Frontiers of RNA and DNA editing", H. Smith, ed., Wiley-Interscience, pp. 190-202. |
Education Background & Academic Experience
2011-present: Principal Investigator, Institute of Biochemistry and Cell Biology, SIBS, CAS, China
2010-2011: Assistant Professor, University of Connecticut Health Center, USA
2009-2010: Post-Doc fellow, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, USA. (Supported by Connecticut Stem Cell Seed Award, PI/Ling-Ling Chen)
2007-2009: M.B.A. in Management, University of Connecticut, USA.
2004-2009: Ph.D. in Biomedical Science, University of Connecticut Health Center, USA
2000-2003: M.S. in Pharmacology, Shanghai Institute of Materia Medica, CAS, China
1996-2000: B.S. in Biology, Lanzhou University, China
Research Team
 |
