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Principal Investigators
State Key Laboratory of Molecular Biology
State Key Laboratory of Cell Biology
Center for Cell Signaling
National Center for Protein Science Shanghai
Members of CAS, CAE
National Outstanding Young Scientists Award
100 Talents Program of the CAS
Address: 320 Yue Yang Road, Shanghai 200031, P.R. China
Tel: 86-21-54920000
Fax: 86-21-54921011
Email: sibcb@sibs.ac.cn
Website: www.sibcb.ac.cn
Principal Investigators
WU Ligang
Ph.D., Professor
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Tel: 54921321
Email: lgwu@@sibcb.ac.cn

Research Areas
Mechanisms of gene expression regulation by microRNA and RNAi

Research Interests

The cells of various eukaryotic organisms contain numerous small and long non-coding RNAs (ncRNA). However, to the majority of those ncRNAs, the questions as basic as whether they have functions and their regulatory mechanisms are still mysterious.  Our previous studies have shown that microRNA (miRNA) destabilize messages to which they are partially complementary by expediting poly(A) tail removal, and further identified CAF1 as the nuclease responsible for mRNA deadenylation mediated by miRNA and off-target RNAi caused by partially complementary siRNA. More recently, we revealed a new function of miRNA in the cells that miRNAs can serve as a surveillance system to repress nonsense mRNAs by recognizing miRNA-responsive elements in the open reading frame region downstream of the premature termination codon. We will continue to focus on discovering novel functions of ncRNAs and elucidating their molecular mechanisms in cell fate determination, which will allow us to develop advanced technology to manipulate gene expression for both basic research and therapeutic applications.
The current projects in this laboratory including:
1)      Studying mechanism of small non-coding RNA in gene regulation.
2)      Developing new strategy to improve the efficiency and specificity of RNAi and CRISPR.
3)      Using high-throughput screening method to identify the function of small and long non-coding RNA in cell fate determination and uncover the molecular mechanism.

Selected Publications

1.       Du H, Zhao Y, He J, Zhang Y, Xi H, Liu M, Ma J* and Wu L*. YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex. Nat Commun. (2016) 7:12626.DOI: 10.1038/ncomms12626
2.       Yang Q, Lin J, Liu M, Li R, Tian B, Zhang X, Xu B, Liu M, Zhang X, Li Y, Shi H*, Wu L*. Highly Sensitive Sequencing Reveals Dynamic Modifications and Activities of Small RNAs in Mouse Oocytes and Early Embryos. Sci Adv. (2016) Vol. 2, no. 6, e1501482 DOI: 10.1126/sciadv.1501482
4.       Liu S, Zhang X, Shi C, Lin J, Chen G, Wu B, Wu L, Shi H, Yuan Y, Zhou W, Sun Z, Dong X, Wang J. Altered microRNAs expression profiling in cumulus cells from patients with polycystic ovary syndrome. J Transl Med. (2015) 13:238. doi: 10.1186/s12967-015-0605-y.
5.       Chen T, Hao Y, Zhang Y, Li M, Wang M, Han W, Wu Y, Lv Y, Hao J, Wang L, Li A, Yang Y, Jin K, Zhao X, Li Y, Ping X, Lai W, Wu L, Jiang G, Wang H, Sang L, Wang X, Yang Y, Zhou Q. m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency. Cell Stem Cell. (2015) 16(3):289-301.
6.       Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L*, Tang F*, Li J*. Correction of a Genetic Disease by CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells.  Cell research. (2015) 25 (1):67-79. 
Insight: ¡°Stop the nonsense!¡±  Elife. (2014) 3:e04300
8.       Gou L, Dai P, Yang J, Xue Y, Hu Y, Zhou Y, Kang J, Wang X, Li H, Hua M, Zhao S, Hu S, Wu L, Shi H, Li Y, Fu X, Qu L, Wang E, Liu M. Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis. Cell research. (2014) 24: 680¨C700.
9.       Piao X, Zhang X, Wu L* and Belasco JG*. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells. Mol Cell Biol. (2010) 30(6): 1486-1494.
11.    Wu L, Fan J, and Belasco JG. Importance of translation and nonnucleolytic ago proteins for on-target RNA interference. Curr Biol. (2008) 18(17): 1327-1332.
12.    Wu L, and Belasco JG. Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay. Methods Enzymol. (2008) 449: 373-393.
13.    Wu L, and Belasco JG. Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAs. Mol Cell. (2008) 29(1): 1-7. (Invited Review)
15.    Wu L, Fan J, and Belasco JG. MicroRNAs direct rapid deadenylation of mRNA. Proc Natl Acad Sci U S A. (2006) 103(11): 4034-4039. 
Commentary: ¡°MicroRNAs: new players in an old game.¡±, PNAS. (2006) 103(11):3951-2.
Research Highlights: ¡°Behind the scenes¡±  Nature Review Genetics, Nature Review Molecular Biology, (2006) 243(7).
16.    Wu L, and Belasco JG. Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells. Mol Cell Biol. (2005) 25(21): 9198-9208.
17.    Wu L, Fan J, Jiang L, Wang H, Song R, Zhang Q, Zhu H, Li N, Liu Z, and Xu Z. A specific cis-hairpin ribozyme facilitates infection of a TMV-based DNA vector in tobacco protoplasts. J Virol Methods. (2003) 111(2): 101-109.
18.    Wu L, Jiang L, Zhou Z, Fan J, Zhang Q, Zhu H, Han Q, and Xu Z. Expression of foot-and-mouth disease virus epitopes in tobacco by a tobacco mosaic virus-based vector. Vaccine. (2003) 21(27-30): 4390-4398.

Education Background & Academic Experience

2008-present:  Principle Investigator, Shanghai Institute of Biochemistry and Cell Biology (SIBCB), CAS, Shanghai, China.
2001-2008:  Postdoctoral Fellow, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA.  
1996-2001:   Ph.D.     in Genetics, National Laboratory of Plant Molecular and Genetics, Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences, Shanghai, China.
1992-1996:   B.S.       in Biochemistry Engineering, Department of Bioscience and Technology, Shanghai Jiao Tong University, Shanghai, China.

Research Team


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