国家蛋白质科学
中心•上海(筹)

分子生物学国
家重点实验室

细胞生物学国
家重点实验室

上海市分子男
科学重点实验室

中国科学院系统
生物学重点实验室
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地址:上海市岳阳路320号
邮编:200031
电话:86-21-54920000
传真:86-21-54921011
邮箱:sibcb@sibs.ac.cn

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李劲松
研究员,课题组长,博士生导师
电话:54921422
E-mail: jsli@@sibcb.ac.cn

个人简介:
        1993年7月毕业于江西农业大学畜牧兽医系,获理学学士学位;1996年7月毕业于扬州大学畜牧兽医学院,获理学硕士学位;2002年7月毕业于中国科学院动物研究所,获理学博士学位;2002年8月至2007年7月在美国洛克菲勒大学从事博士后研究;2007年8月起任中科院上海生科院生物化学与细胞生物学研究所研究员,研究组长。2008年获“百人计划”项目支持,2012年获“杰出青年基金”支持。

研究方向:体细胞重编程与诱导多能干细胞

研究工作:
       

体细胞重编程(somatic reprogramming)指的是分化的体细胞在特定的条件下被逆转后恢复到全能性状态或多能性状态,或者形成多能干细胞系,或者进一步发育成一个新的个体的过程。诱导体细胞重编程的方法有许多,如核移植、细胞融合、细胞提取物诱导、化学诱导以及诱导多能干细胞(induced pluripotent stem cell, iPSC)技术等。但到目前为止,能诱导体细胞产生具有功能的重编程的方法只有核移植和iPSC技术,其他的方法只能在细胞、分子或生化水平上产生诱导。然而所有这些诱导体细胞重编程的技术都很不成熟,效率很低,体细胞重编程的机制还不清楚。虽然如此,体细胞重编程却有着非常广泛的应用前景,不仅在基础理论研究中,而且在应用研究中,例如家畜繁殖、动物保护以及人口健康等领域都有着重要的作用。因此,研究体细胞重编程的机制,提高重编程的效率以及开展体细胞重编程的应用研究显得尤为必要和紧迫。
        本实验室将开展以下几方面的研究:
        (1)完善核移植及iPSC技术诱导体细胞重编程的方法,提高体细胞重编程的效率;
        (2)探讨核移植与iPSC技术诱导体细胞重编程的机制;
        (3)建立新的诱导体细胞重编程的方法;
        (4)开展体细胞重编程的应用研究。

代表性论文:

  1. Guo F#, Li X#, Liang D#, Li T#, Zhu P, Guo H, Wu X, Wen L, Gu T, Hu B, Walsh CP, Li Jinsong*, Tang F* & Xu G*. Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote. Cell Stem Cell 2014 [Epub ahead of ]
  2. Zhang M, Zhou H, Zheng C, Xiao J, Zuo E, Liu W, Xie D, Shi Y, Wu C, Wang H, Li D & Li Jinsong. The roles of testicular c-kit positive cells in de novo morphogenesis of testis. Sci Rep 2014, 4: 5936.
  3. Zhu Q, Song L, Peng G, Sun N, Chen J, Zhang T, Sheng N, Tang W, Qian C, Qiao Y, Tang K, Han JD, Li Jinsong & Jing N. The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. Elife 2014, Jun 14:e02224. doi: 10.7554/eLife.02224. [Epub ahead of print].
  4. Zhao B, Yang D, Jiang J, Li Jinsong, Fan S, Huang M, Fan Y, Jin Y & Jin Y. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies. BMC Genomic 2014, 15: 488.
  5. Yu Y, Liang D, Tian Q, Chen X, Jiang B, Chou BK, Hu P Cheng L, Gao P, Li Jinsong & Wang G. Stimulation of somatic cell reprogramming by Eras-Akt-Foxo1 signaling axis. Stem Cells 2014, 32(2): 349-363. doi: 10.1002/stem.1447. [Epub ahead of print].
  6. Qin Y, Zhou P, Zhou C, Li Jinsong & Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev 2014, 23: 363-371.
  7. Wu Y#, Liang D#, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D & Li Jinsong*. Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell 2013, 13: 659-662.
  8. Yang H#, Liu Z#, Ma Y#, Zhong C, Yin Q, Zhou C, Shi L, Cai Y, Zhao H, Wang H, Tang F, Wang Y, Zhang C, Liu X, Lai D, Jin Y*, Sun Q* & Li Jinsong*. Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. Cell Res 2013, 23: 1187-1200.
  9. Qin Y#, Lin J#, Zhou C#, Yin Q, Xie Z, Zhang X, Liu X, Gao W & Li Jinsong*. Mice cloned from white adipose tissue-derived cells. J Mol Cell Biol 2013, 5: 348-350.
  10. Jiang J#, Lv W#, Ye X, Wang L, Zhang M, Yang H, Okuka M, Zhou C, Zhang X, Liu L* & Li Jinsong*. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrasted by tetraploid complementation. Cell Res 2013, 23: 92-106.
  11. Zhao S#, Gou LT#, Zhang M#, Zu LD, Hua MM, Hua Y, Shi HJ, Li Y, Li Jinsong, Li DS, Wang ED* & Liu M. piRNA-triggered MIWI ubiquitination and removal by APC/C in late spermatogenesis. Dev Cell 2013, 24: 13-25.
  12. Yang H#, Shi L#, Wang B#, Liang D, Zhong C, Liu W, Nie Y, Liu J, Zhao J, Gao X, Li D, Xu G-L* & Li Jinsong*. Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. Cell 2012, 149(3): 605-617.
  13. Shi L, Yang H & Li Jinsong*. Haploid embryonic stem cells: an idea tool for mammalian genetic analyses. Protein & Cell 2012, 3: 806-810. (Invited review)
  14. Ma Y, Gu J, Li C, Wei X, Tang F, Shi G, Jiang J, Kuang Y, Li Jinsong, Wang Z, Xie X & Jin Y. Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells. Stem Cell Res Ther 2012, 3: 29.
  15. Gu TP#, Guo F#, Yang H#, Wu HP, Xu GF, Liu W, Xie ZG, Shi L, He X, Jin SG, Iqbal K, Shi YG, Deng Z, Szabo PE, Pfeifer GP, Li Jinsong* & Xu GL*. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 2011, 477, 606-610.
  16. Jiang J#, Ding G#, Lin J#, Zhang M, Shi L, Lv W, Yang H, Xiao H, Pei G, Li Y, Wu J* & Li Jinsong*. Different developmental potential of pluripotent stem cells generated by different reprogramming strategies. J Mol Cell Biol 2011, 3: 197-199.
  17. Lin J, Shi L, Zhang M, Yang H, Qin Y, Zhang J, Gong D, Zhang X, Li D & Li Jinsong*. Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer. Cell Stem Cell 2011, 8: 371-375.
  18. Yang H#, Shi L#, Chen D & Li Jinsong*. Mice generated after round spermatid injection into haploid two-cell blastomeres. Cell Res 2011, 21: 854-858.
  19. Li X, Zhu L, Yang A, Lin J, Tang F, Jin S, Wei Z, Li Jinsong & Jin Y. Calcineurin-NFAT signaling critically regulates early lineage specification in mouse embryonic stem cells and embryos. Cell Stem Cell 2011 8: 46-58.
  20. Wang Y, Chen J, Hu J, Wei X, Qin D, Gao J, Zhang L, Jiang J, Li Jinsong, Liu J, Lai K, Kuang X, Zhang J, Pei D & Xu G. Reprogramming of mouse and human somatic cells by high-performance engineered factors. EMBO Rep 2011, 12: 373-378.
  21. Yang Hui, Shi Linyu, Zhang Shenghua, Ling Jiangwei, Jiang Jing & Li Jinsong*. High-efficiency somatic reprogramming induced by intact MII oocytes. Cell Res 2010, 20, 1034-42.
  22. Li L, Sun L, Gao F, Jiang J, Li C, Gu J, Wei Z, Yang A, Lu R, Ma Y, Tang F, Kwon SW, Zhao Y, Li Jinsong & Jin Y. Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation. Proc Natl Acad Sci USA 2010, 107(4):1402-1407.
  23. Chen T, Yuan D, Wei B, Jiang J, Kang J, Ling K, Gu Y, Li Jinsong, Xiao L & Pei G. E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation. Stem Cells 2010, 28: 1315-1325.
  24. Zhu Z, Wang Y, Li X, Wang Y, Xu L, Wang X, Sun T, Dong X, Chen L, Mao H, Yu Y, Li Jinsong, Chen PA & Chen CD. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis. Cell Res 2010, 20: 794-801.
  25. Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, Yang Y, Jin S, Wei Z, Jiang H, Li Jinsong & Jin Y. Germliine-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor. PLos One 2009, 4: e6724.
  26. Huang J, Chen T, Liu X, Jiang J, Li Jinsong, Li D, Liu X, Li W, Kang J & Pei G. More synergetic cooperation of Yamanaka factors in induced pluripotent stem cells than in embryonic stem cells. Cell Res 2009, 19: 1127-1138.
  27. Li Jinsong & Mombaerts P. Nuclear transfer-mediated rescue of the nuclear genome of nonviable mouse cells frozen without cryoprotectant. Biol Reprod 2008 79(4): 588-593.
  28. Li Jinsong#, Guasch Geraldine#, Greco Valentina#, Fuchs Elaine* & Mombaerts P*. Cloned mice from Skin Stem Cells. Proc Natl Acad Sci USA 2007, 104(8): 2738-2743.
  29. Li Jinsong, Tomohiro Ishii, Duancheng Wen & Mombaerts P. Non-equivalence of cloned and clonal mice. Current Biology 2005, 15(18): R756-R757. 
  30. Li Jinsong, Tomohiro Ishii, Paul Feinstein & Mombaerts P. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 2004, 428: 393-398.
  31. Han Z, Chen D, Li Jinsong, Sun Q, Wan Q, Kou Z, Rao G, Lei L, Liu Z & Fang S.  Mitochondrial DNA heteroplasmy in calves cloned by using adult somatic cell. Mol Reprod Dev 2004, 67(2): 207-214
  32. Chen D#, Li Jinsong#, Han Z, Lei L, Liu Z, Kou Z, Ma S, Du Q & Sun Q. Somatic cell bovine cloning: efficiency of donor cells from female and male using different recipient cows. Chinese Science Bulletin 2003, 48(6): 549-554.
  33. Zhu Z, Chen D, Li Jinsong, Lian L, Lei L, Han Z & Sun Q. Rotation of meiotic spindle is controlled by microfilaments in mouse oocytes. Biology of Reproduction 2003, 68: 943-946.
  34. Han Z, Chen D, Li Jinsong, Sun Q, Wang P, Du J & Zhang H. Flow cytometric cell-cycle analysis of cultured fibroblasts from the giant panda, Ailuropoda melanoleuca L. Cell Biol Int 2003, 27(4): 349-353
  35. Wen D, Yang C, Cheng Y, Li Jinsong, Liu Z, Sun Q, Zhang J, Lei L, Wu Y, Kou Z & Chen D. Comparison of developmental capacity for intra- and interspecies cloned cat (Felis catus) embryos. Mol Reprod Dev 2003, 66(1): 38-45
  36. Chen Y, He Z, Liu A, Wang K, Mao W, Chu J, Lu Y, Fang Z, Shi Y, Yang Q, Chen D, Wang M, Li Jinsong, Huang S, Kong X, Shi Y, Wang Z, Xia J, Long Z, Xue Z, Ding W & Sheng H. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.  Cell Res 2003, 13(4): 251-263.
  37. Li Jinsong, Chen D, Han Z, Zhu Z, Wen D, Liu Z, Wang M, Lian L, Du J, Wang P & Zhang H. Serial Nuclear Transfer Improves the Development of Interspecies Reconstructed Giant Panda Embryos. Chinese Science Bulletin 2002, 47(6): 467-469
  38. Chen D, Wen D, Zhang Y, Sun Q, Han Z, Liu Z, Shi P, Li Jinsong, Xianyu J, Lian L, Kou Z, Wu Y, Chen Y, Wang P & Zhang H. Interspeices implantation and mitochondria fate of panda-rabbit cloned embryos. Biol Reprod 2002, 67: 637-642.
  39. Han Z, Chen D, Li Jinsong, Sun Q, Wang P, Huang Y & Du J. The culture of fibroblasts from diaphragm of Giant Panda. In Vitro Cell Dev Biol 2001, 37: 644-645
  40. Li G, Chen D, Lian L, Sun Q, Wang M, Liu J, Li Jinsong & Han Z. Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplamic sperm injection (ICSI). Mol Reprod Dev 2001, 58: 180-185
  41. Ding B, Shi P, Xianyu J, Zhang Y, Chen D, Sun Q, Li G, Wang M, Liu J, Han Z, Song X, Li Jinsong & Chen Y. Microsatellite DNA analysis prove nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda. Chinese Science Bulletin 2000, 45(20): 1883-1885
  42. Liu J, Wang M, Lian L, Li jinsong, Gao S, Han Z, Sun Q, Song X, Zhang D, Li Y, Xu Z & Chen D. Nuclear Transfer Using Nonquiescent Adult Fibroblasts from a Bovine Ear. Chinese Science Bulletin 1999, 44(21): 1971-1974
  43. Chen D, Sun Q, Liu J, Li G, Lian L, Wang M, Han Z, Song X, Li Jinsong, Sun Q, Chen Y, Zhang Y & Ding B. The Giant Panda Somatic Nucleus can dedifferentiate in Rabbit Ooplasm and Support Early Development of the Reconstructed Egg. Science in China 1999, 42(4): 346-353

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