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院士风采
杰出青年
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地址:上海市岳阳路320号
邮编:200031
电话:86-21-54920000
传真:86-21-54921011
邮箱:sibcb@sibcb.ac.cn

全所PI名录 >

 
陈勇
研究员,研究组长,博士生导师
E-mail: yongchen@@sibcb.ac.cn


个人简介

1999年毕业于清华大学生物科学与技术系,获得生物学学士学位。2005年1月获清华大学生物物理学博士学位;2005年—2012年在美国密西根大学医学院生物化学系从事博士后研究。2012年起任中科院上海生科院生化与细胞所研究员,研究组长,博士生导师。


研究方向

表观遗传学的结构生物学研究


研究工作
真核生物染色体不仅是遗传信息的载体,也是一系列重要生物学过程的发生地点。染色体的局部构象改变和整体的状态变化,和基因表达,转录,损伤修复,重组等息息相关。染色体的基本组成单位是由组蛋白和DNA组成核小体,核小体的进一步折叠可以形成染色质的高级结构。
        多年来的研究表明组蛋白化学修饰、DNA甲基化、依赖ATP的染色体重塑、组蛋白变体以及非编码RNA等表观遗传因子在染色质高级结构的建立和维持中起着重要的调控作用。它们是如何在染色体的结构调节和基因转录调控中协同发挥作用的,是目前生物学研究的热点。而且若干表观遗传因子的突变或缺失和很多疾病的发生有密切联系。
        本实验室的主要研究方向将集中在重要表观遗传因子的结构生物学研究上。我们将对一些关键的表观遗传因子复合物利用结构生物学和生物化学的手段进行深入的研究,以揭示其活性机制,调节其机理和设计针对其特定结构靶点的药物小分子。具体研究内容包括: 揭示依赖ATP的染色体重塑复合物的分子作用机制;阐明依赖ATP的染色体重塑与组蛋白修饰之间的协同作用机制; 阐析端粒染色体组装的分子机制。


代表性论文

  1. Yong Chen, Fang Cao, Bingbing, Yali Dou, Ming Lei (2011) Structure of the SPRY domain of Ash2L and its interactions with RbBP5 and DPY30, Cell Res., 22, 598-602
  2. Yong Chen, Bingbing Wan, Kevin C. Wang, Fang Cao, Yuting Yang, Angeline Protacio, Yali Dou, Howard Y. Chang, Ming Lei (2011) Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding, EMBO Rep. 12(8),797-803
  3. Kevin Wang, Yul Yang, Rajnish Gupta, Angeline Protacio, Bo Liu, Jill Helms, Yong Chen, Ming Lei, Joanna Wysocka, Amartya Sanyal, Bryan Lajoie, Job Dekker, M. Corces-Zimmerman, Howard Chang (2011) A long noncoding RNA maintains active chromatin to coordinate homeotic gene express, Nature, 472(7341), 120-124
  4. Ying Tang, Xijiang Pan, Yong Chen, Phang C. Tai, Sen-Fang Sui (2011) Dimeric SecA couples the preprotein translocation in an asymmetric manner. PLoS One, 6(1), e16498
  5. Yong Chen, Rekha Rai, Zi-Ren Zhou, Junko Kanoh, Cyril Ribeyre, Yuting Yang, Hong Zheng, Pascal Damay, Feng Wang, Hisayo Tsujii, David Shore, Hong-Yu Hu, Sandy Chang A, Ming Lei (2011) A Conserved Motif within RAP1 Plays Diversified Roles in Telomere Protection and Regulation in Different Organisms, Nat. Struct. Mol. Biol., 18(2): 213-221
  6. Fang Cao, Yong Chen, Tomasz Cierpicki, Yifan Liu, Venkatesha Basrur, Ming Lei, Yali Dou (2010) An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain. PLoS One 5(11), e14102
  7. Zhixiong Zeng, Wei Wang, Yuting Yang, Yong Chen, Xiaomei Yang, J.Alan Diehl, Xuedong Liu and Ming Lei (2010) Structural basis of selective ubiquitination of TRF1 bys SCFFBX4. Dev. Cell, 18, 214-225
  8. Yong Chen, Yuting Yang, Megan van Overbeek, Jill R. Donigian, Paul Baciu, Titia de Lange, Ming Lei (2008) A Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins. Science, 319: 1092-1096
  9. Yong Chen, Xijiang Pan, Ying Tang, Shu Quan, Phang C. Tai, Sen-Fang Sui (2008) Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryo-electron microscopy. J. Biol. Chem. 283, 28783-28787
  10. Jiansen Jiang, Xuefeng Zhang, Yong Chen, Yi Wu, Z. Hong Zhou, Zengyi Chang, Sen-Fang Sui (2008) Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins. Proc. Natl. Acad. Sci. U S A. 105: 11939-11944
  11. Yong Chen, Phang C. Tai, Sen-Fang Sui (2007) The active ring-like structure of SecA revealed by electron crystallography: conformational change upon interaction with SecB. J. Struct. Biol. 159: 149-153
  12. Yong Chen, Yuting Yang, Feng Wang, Ke Wan, Kenichi Yamane, Yi Zhang, Ming Lei (2006) Crystal structure of human histone lysine-specific demethylase 1 (LSD1). Proc. Natl. Acad. Sci. U S A. 103:13956-13961
  13. Shi-Zhen Wang, Yong Chen, Zhan-Hua Sun, Qiang Zhou, Sen-Fang Sui (2006) Escherichia coli CorA periplasmic domain functions as a homotetramer to bind substrate. J. Biol. Chem. 281: 26813-26820
  14. Chang-Zhen Liu, Yong Chen and Sen-fang Sui, (2006) The identification of a new actin-binding region in p57. Cell Res., 16, 106-112
  15. Hong-Wei Wang, Yong Chen, Hsiuchin Yang, Xianchuan Chen, Ming-Xing Duan, Phang C. Tai and Sen-fang Sui,Wang(2003) Ring-like pore structures of SecA: implication for bacterial protein-conducting channels. Proc. Natl. Acad. Sci. U S A. 100, 4221-4226
  16. Yong Chen, Ying-Jie Lu, Hong-Wei Wang, Shu Quan, Zengyi Chang and Sen-fang Sui, Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layer, Biochem. Biophys. Res. Commun., 2003, 310: 360-366
  17. Yong Chen, Jinmei Song, Sen-fang Sui and Da-Neng Wang, DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli, Protein Expr. Purif., 2003, 32:221-231
  18. Hong-Wei Wang, Yi Wu, Yong Chen and Sen-Fang Sui(2002)Polymorphism of structural forms of C-reactive protein. Int. J. Mo.l Med. 9:665-671

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