人才队伍

情况简介
院士风采
杰出青年
全所PI名录
 
 
地址:上海市岳阳路320号
邮编:200031
电话:86-21-54920000
传真:86-21-54921011
邮箱:sibcb@sibcb.ac.cn

全所PI名录 >

 
陈玲玲
研究员,研究组长,博士生导师
E-mail: linglingchen@@sibcb.ac.cn
Lab homepage: www.chenlab-ncrna.com


个人简介

2009年毕业于美国康涅狄格大学医学院获生物医学博士学位,同时获商学院工商管理学硕士学位。同年5月作为独立PI获得Connecticut Stem Cell Seed Award研究经费资助,受聘于康涅狄格大学干细胞学院从事博士后研究,并于2010年5月任助理教授。2011年1月任上海生化与细胞所研究员。目前担任国际期刊Trends in geneticsGenome biologyJournal of Biological Chemistry编委。2017年入选为霍华·德休斯医学院研究所(HHMI)国际研究员、入选国家杰出青年科学基金,获谈家桢生命科学创新奖;2016年入选全球华人生物学家协会CBIS杰出青年研究员奖、中国青年女科学家奖等荣誉。


研究方向

长非编码RNA和干细胞


研究工作
        人类基因组测序计划发现多于98%的转录基因组序列为非编码RNA(ncRNA)。这些ncRNA包括人们所熟知的housekeepingncRNA(如tRNAs, rRNAs和snRNAs等)、小RNA(如miRNAs和piRNAs等),以及上万条长非编码RNA(lncRNA,>200nt)。最近几年来的研究表明lncRNAs广泛参与一系列细胞的重要功能调控,包括细胞核亚结构的形成、基因表达的遗传与表观遗传调控等。
        我们实验室主要研究哺乳动物细胞中lncRNAs的功能调控,包括新类型lncRNA分子的发掘、其加工成熟机制、以及它们在转录、染色体、细胞核亚结构和干细胞干性维持等方面的调控功能研究。未来五年,我们主要研究以下内容:
        1、通过转录组测序和计算生物学手段结合的方法,发掘和鉴定人类基因组中的新类型lncRNA分子
        2、利用分子生物学、细胞生物学和生物化学的手段,研究新lncRNA分子在基因表达调控中的作用机制
        3、采用基因组编辑和活细胞成像等技术手段,研究lncRNA在细胞核亚结构和染色体维持和调控过程中的功能
        4、以hESCs的多功能潜能性维持和分化为模型,研究lncRNA对人源胚胎干细胞的命运决定的调控
        研究成果将能够丰富我们对lncRNAs功能和机制的认识,也为hESCs的多功能性维持和命运改变提供一个全新水平的调控。


代表性论文

  1. Liu CX#, Li X#, Nan F#, Jiang S#, Gao X, Guo SK, Xue W, Cui YG, Dong KG, Ding HH, Qu B, Shen N*, Yang L*, Chen LL*. 2019. Structure and degradation of circular RNAs regulate PKR activation in innate immunity. Cell, 177:865-880. (preview by Jeremy E. Wilusz, Cell, 2019, 177:797-799; research highlights by Science New, Nat Rev Immunol, Nat Rev Mol Cell Biol and Cell Biosci; recommendation in F1000 Prime)
  2. Yao RW, Wang Y and Chen LL*. 2019. Cellular functions of long noncoding RNAs. Nat Cell Biol,  21:542-551. (Editorial by Nat Cell Biol, 2019, 21: 535.)
  3. Wang Y#, Hu SB#, Wang MR#, Yao RW, Wu D, Yang L and Chen LL*. 2018. Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria.Nat Cell Biol, 20:1145-1158. (Editorial by Fox AH. Nat Cell Biol, 2018, 20: 1108-1109.)
  4. Li X, Yang L* and Chen LL*. 2018. The biogenesis, functions and challenges of circular RNAs.Mol Cell, 71:428-442.
  5. Xiang JF#, Yang Q#, Liu CX#, Wu M, Chen LL* and Yang L*. 2018. N6-methyladenosines modulate A-to-I RNA editing. Mol Cell, 69:126-135.
  6. Yang L* and Chen LL*. 2017. Enhancing the RNA engineering tool kit. Science, 358: 996-997.
  7. Li X#, Liu CX#, Xue W#, Zhang Y, Jiang S, Yin QF, Wei J, Yao RW, Yang L* and Chen LL*. 2017. Coordinated circRNA biogenesis and function with NF90/NF110 in viral infection. Mol Cell, 67: 214-222. (Free featured article; highlighted by Editor’s Note and Meet the Author; preview by Cadena CY and Hur S. Mol Cell, 2017, 67:163-164.)
  8. Wu H, Yang L*, Chen LL*. 2017. The diversity of long noncoding RNAs and their generation. Trends Genet, 33:540-552.
  9. Xing YH#, Yao RW#, Zhang Y#, Guo CJ, Jiang S, Xu G, Dong R, Yang L, Chen LL*. 2017. SLERT regulates DDX21-rings associated with Pol I transcription. Cell, 169: 664-678. (Research highlights by Bull Chin Acad Sci, 2017, 31:152-155.)
  10. Dong R#, Ma XK#, Chen LL* and Yang L*. 2017. Increased complexity of circRNA expression during species evolution. RNA Biol, 14:1064-1074.
  11. Chen LL* and Yang L*. 2017. AlUternative regulation for gene expression. Trends Cell Biol, 27:480-490.
  12. Wu H#, Yin QF#, Luo Z#, Yao RW, Zheng CC, Zhang J, Xiang JF, Yang L and Chen LL*. 2016. Unusual processing generates SPA lncRNAs that sequester multiple RNA binding proteins. Mol Cell, 64:435-437. (Cover story and Issue highlights; preview by Li Y and Fox AH. Mol Cell, 2016, 64:435-437.)
  13. Xing YH#, Bai ZQ#, Liu CX, Hu SB, Ruan MH and Chen LL*. 2016. Research progress of long noncoding RNA in China. IUBMB Life, 68:887-893.
  14. Hu SB, Yao RW and Chen LL*. 2016. Shedding light on paraspeckle structure by super-resolution microscopy. J Cell Biol, 214:789-791.
  15. Chen LL*. 2016. Linking long noncoding RNA localization and function. Trends Biochem Sci, 41:761-772. (Series: Fresh Perspectives from Emerging Experts)
  16. Zhang XO#, Dong R#, Zhang Y#, Zhang JL, Luo Z, Zhang J, Chen LL*and Yang L*. 2016. Diverse alternative back-splicing and alternative splicing landscape of circular RNAs. Genome Res, 26: 1277-1287.
  17. Zhang Y#, Xue W#, Li X, Zhang J, Zhang JL, Yang L* and Chen LL*. 2016. The biogenesis of nascent circular RNAs. Cell Rep, 15:611-624.
  18. Chen LL*. 2016. The biogenesis and emerging roles of circular RNAs. Nat Rev Mol Cell Biol, 17:205-211. (Cover story)
  19. Zhang Y, Yang L and Chen LL*. 2016. Characterization of circular RNAs. Methods Mol Biol, 1402:215-27.
  20. Xiang JF, Yang L and Chen LL*. 2015. The long noncoding RNA regulation at the MYC locus. Curr Opin Genet Dev, 33:41-48.
  21. Chen LL* and Yang L*. 2015. Gear up in circles. Mol Cell, 58:715-717.
  22. Chen LL* and Yang L*. 2015. Regulation of circRNA biogenesis. RNA Biol, 12:381-388.
  23. Hu SB, Xiang JF, Li X, Xu YF, Xue W, Huang M, Wong CC, Sagum CA, Bedford MT, Yang L, Cheng D* and Chen LL*. 2015. Protein arginine methyltransferase CARM1 attenuates the nuclear retention of mRNAs containing IRAlus at paraspeckles. Genes Dev, 29: 630-645. (Perspectives by Elbarbary RA and Maquat LE, Genes Dev, 2015, 29:687–689; research highlights by Bull Chin Acad Sci, 2015, 29:108)
  24. Chen T#, Xiang JF#, Zhu S#, Chen S, Yin QF, Zhang XO, Zhang J, Feng H, Dong R, Li XJ, Yang L* and Chen LL*. 2015. ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner. Cell Res, 25:459-476.
  25. Yin QF#, Hu SB#, Xu YF, Yang L, Carmichael GG and Chen LL*. 2015. SnoVectors for nuclear expression of RNA. Nucleic Acids Res, 43(1):e5.
  26. Yang L* and Chen LL*. 2014. Microexons go big. Cell, 159:1488-1489.
  27. Zhang XO#, Wang HB#, Zhang Y, Lu X, Chen LL* and Yang L*. 2014. Complementary sequence- mediated exon circularization. Cell, 159:134-147. (Featured article; preview by Vicens Q and Westhof E, Cell, 2014,159:13-14; research highlights by Nat Rev Genetics, 2014, 15:717; F1000 Prime recommendation and Bull Chin Acad Sci, 2014, 28:282-284)
  28. Zhang Y, Yang L and Chen LL*. 2014. Life without A tail: new formats of long noncoding RNAs. Int J Biochem Cell Biol, 54:338-349.
  29. Zhang XO #, Yin QF #, Wang HB, Zhang Y, Chen T, Zheng P, Lu X, Chen LL* and Yang L*. 2014. Species-specific alternative splicing leads to unique expression of sno-lncRNAs. BMC Genomics, 15:287. (Highly accessed; featured article in Biome)
  30. Xiang JF, Yin QF, Chen T, Zhang Y, Zhang XO, Wang HB, Ge JH, Lu XH, Yang L and Chen LL*. 2014. Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions in the MYC locus. Cell Res, 24:513-531. (Cover article; research highlights by Younger ST and Rinn JL. Cell Res, 2014, 24:643-644; research highlights by Nature.com, Global Medical Discovery and National Science Review, 2015, 2:7-8.)
  31. Chen LL* and Zhao JC*. 2014. Functional study of long noncoding RNAs in development and diseases. Adv Exp Med Biol, 825:129-158.
  32. Zhu S#, Xiang JF#, Chen T, Chen LL* and Yang L*. 2013. Prediction of constitutive A-to-I editing sites from human transcriptomes in the absence of genomic sequences. BMC Genomics, 14:206.
  33. Zhang Y#, Zhang XO#, Chen T, Xiang JF, Yin QF, Xing YH, Zhu S, Yang L* and Chen LL*. 2013. Circular intronic long noncoding RNAs, Mol Cell, 51: 792-806. (Preview by Bolisetty MT and Graveley BR. Mol Cell, 2013, 51:705-706; research highlights by Nature, 2013, 501:464, Nature China, 2013, doi:10.1038; the most- downloaded article of the month in Mol Cell)
  34. Yin QF#, Yang L#, Zhang Y, Xiang JF, Wu YW, Carmichael GG* and Chen LL*. 2012. Long noncoding RNAs with snoRNA ends. Mol Cell, 48: 219-230. (Cover article; preview by McCann KL and Baserga SJ. Mol Cell, 2012, 48:155-7; research highlights by Nat Rev Mol Cell Biol, 2012, 13:686; F1000 Prime recommendation; news coverage in Foundation for Prader-Willi Research; Best of Mol Cell 2012)
  35. Yang L, Duff MO, Graveley BR, Carmichael GG and Chen LL*. 2011. Genomewide characterization of non- polyadenylated RNAs. Genome Biol, 12: R16. (Editor's pick; highly accessed)
  36. Chen LL* and Carmichael GG. 2010. Long non-coding RNAs in mammalian cells: What, Where and Why? WIREs RNA, 1: 2-21.
  37. Chen LL* and Carmichael GG*. 2010. Decoding the function of nuclear long non-coding RNAs. Curr Opin Cell Biol, 22: 357-364.
  38. Chen LL*, Yang L and Carmichael GG*. 2010. Molecular basis for an attenuated cytoplasmic dsRNA response in human embryonic stem cells. Cell Cycle, 9:3552-3564. (Research highlights by Williams BRG. Cell Cycle, 2010, 35:3400)
  39. Chen LL and Carmichael GG. 2009. Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA. Mol Cell, 35: 467-478. (Preview by Scadden D. (2009) Mol Cell, 35:395-396; F1000 Prime recommendation)
  40. Chen LL and Carmichael GG. 2008. Gene regulation by SINES and inosines: biological consequences of A-to-I editing of Alu element inverted repeats. Cell Cycle, 7: 3297-3301.
  41. Chen LL, Decerbo JN and Carmichael GG. 2008. Alu element-mediated gene silencing. EMBO J, 27: 1694-1705.  

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