人才队伍

情况简介
院士风采
杰出青年
全所PI名录
 
 
地址:上海市岳阳路320号
邮编:200031
电话:86-21-54920000
传真:86-21-54921011
邮箱:sibcb@sibcb.ac.cn

全所PI名录 >

 
戚正武
研究员,中科院院士,博士生导师
E-mail: zwqi@@sibs.ac.cn


个人简介

1932年4月生。1952年毕业于同济大学化学系,1959年获苏联莫斯科医学科学院生物与医学研究所副博士学位。1981-1982年以及1987年、1995年分别于美国Purdue 及Notre Dame大学进行学术交流。曾担任数届国际《血栓研究》杂志的编委,现任《中国科学》,《生物化学与生物物理学报》及其他国内多种学术期刊的编委。现兼同济大学蛋白质研究所所长及化学系主任。1999年被评为中国科学院院士,2001年被评为第三世界科学院院士。曾获中科院自然科学一等奖一次,二等奖三次,国家自然科学三等奖二次。1981年慕尼黑国际激肽会议获优秀论文奖及其他省、部级多次科技奖。1993年曾被评为中国科学院优秀研究生导师。


研究方向

活性多肽及酶蛋白的结构功能


研究工作
长期以来从事活性多肽及酶蛋白结构与功能的研究,利用蛋白质工程技术研究结构与功能的关系。有关蛋白酶抑制剂,蝎及芋螺多肽神经毒素以及与凝血纤溶有关的蛋白质的研究居国际前沿。曾从不同植物中分离纯化了三种不同类型的蛋白酶抑制剂,阐明了它们的蛋白质、基因及晶体结构,确定了活性中心位置,其中多功能双头慈菇蛋白酶抑制剂是属于新的抑制剂家族。利用多肽合成,蛋白酶限止性降解及蛋白质工程等手段研究了它们的结构与功能的关系。在基因水平上阐明了异常人血清白蛋白及血小板无力症遗传性疾病的基因缺陷。对凝血因子Ⅷ及vWF因子进行了基因克隆,并在哺乳动物细胞中获得表达,申请了生产重组凝血因子Ⅷ的专利。90年代以来又开展研究蝎及芋螺毒腺中各种离子通道及神经递质受体的神经毒素,发现了多种结构全新的神经毒素。此外在猪肝中分离纯化了与体内重要生理功能相关的天然蛋白质前体加工酶的抑制剂。


代表性论文

  1. Yuan DD, Liu L, Shao XX, Peng C, Chi CW, Guo ZY. Isolation and cloning of a conotoxin with a novel cysteine pattern from Conus caracteristicus. Peptides. 2008 Sep;29(9):1521-5. Epub 2008 May 25.
  2. Wang Q, Jiang H, Han YH, Yuan DD, Chi CW. Two different groups of signal sequence in M-superfamily conotoxins. Toxicon. 2008 Apr;51(5):813-22. Epub 2007 Dec 15.
  3. Qian J, Guo ZY, Chi CW. Cloning and isolation of a Conus cysteine-rich protein homologous to Tex31 but without proteolytic activity. Acta Biochim Biophys Sin (Shanghai). 2008 Feb;40(2):174-81.
  4. Rui-feng Qi, Ling Zhang, and Cheng-wu Chi. Biological characteristics of dengue virus and potential targets for drug design. Acta Biochim Biophys Sin. 2008 Feb;40(2):91-101.
  5. Wang ZQ, Han YH, Shao XX, Chi CW, Guo ZY. Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus. FEBS J. 2007 Sep;274(18):4778-87. Epub 2007 Aug 14.
  6. Yuan DD, Han YH, Wang CG, Chi CW. From the identification of gene organization of alpha conotoxins to the cloning of novel toxins. Toxicon. 2007 Jun 15;49(8):1135-49. Epub 2007 Mar 1.
  7. Du WH, Han YH, Huang FJ, Li J, Chi CW, Fang WH. Solution structure of an M-1 conotoxin with a novel disulfide linkage. FEBS J. 2007 May;274(10):2596-602. Epub 2007 Apr 16.
  8. Han YH, Wang Q, Jiang H, Liu L, Xiao C, Yuan DD, Shao XX, Dai QY, Cheng JS, Chi CW. Characterization of novel M-superfamily conotoxins with new disulfide linkage. FEBS J. 2006 Nov;273(21):4972-82. Epub 2006 Oct 17.
  9. Chen-Qi Xu, Shun-Yi Zhu, Cheng-Wu Chi and Jan Tytgat. Turret and pore block of K+Channels: what is the different? Trends Pharmac. Sci. 24(9): 446-449, 2003.
  10. Fan CX, Chen XK, Zhang C, Wang LX, Duan KL, He LL, Cao Y, Liu SY, Zhong MN, Ulens C, Tytgat J, Chen JS, Chi CW, and Zhou Z. A novel conotoxin from conus betulinus, k-BtX, unique in cysteine pattern and in function as a specific BK channel modulator. J Biol Chem. 278(15), 12624-12633, 2003.
  11. Wang CG, Gilles N, Hamon A, Le Gall F, Stankiewicz M, Pelhate M, Xiong YM, Wang DC and Chi CW. Exploration of the functional site of a scorpion alpha-like toxin by site-directed mutagenesis. Biochemistry. 42 (16), 4699-4708, 2003.
  12. Wang CG, Stankiewicz M, Ling MH, Pelhate M, Wang DC and Chi CW*. Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch. J. Pept. Res. 61(1): 7-16, 2003.
  13. C. Goudet, Cheng-wu Chi, J Tytgat. An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon. 40(9), 1239, 2002.
  14. Jiong Li, Kang-cheng Ruan, Cheng-wu Chi. The assignment of the reactive sites of the double-headed arrowhead proteinase inhibitor A and B. Acta Biochi. Biophys Sinica. 34, 662, 2002.
  15. Jiong Li, Cheng-wu Chi, Kang-cheng Ruan. Conformation nearby Trp residues of APIA and APIB modulates the inhibitory specificity of the protease. Acta Biochi. Biophys Sinica. 34, 494, 2002.
  16. Jing-Jiang Wu, Lin-Lin He, Zhuan Zhou and Cheng-Wu Chi. Gene expression, mutation and structure-function relationship of scorpion toxin BmP05 active on SKCa channels. Biochemistry. 41, 2844, 2002
  17. Chun Chen, Qi Wang, Xiang-dong Fang, Qin Xu, Cheng-wu Chi and Jian-xin Gu. Roles of the phytanoyl CoA ?-hydroxylase in mediating the expression of human coagulation factor VIII. J. Biol. Chem. 276, 46340, 2001.
  18. Guang-yu Zhu, Qi-chen Huang, Yan-shi Zhu, Cheng-wu Chi, You-qi Tang. X-ray study on an artificial mung bean inhibitor complex with bovine ?-trypsin in neat cyclohexane. Biochim. Biophys. Acta 1546, 98, 2001.
  19. Chun-Guang Wang, Xiao-Lin He, Feng Shao, Wei Liu, Min-Hua Ling, Da-Cheng Wang, Cheng-Wu Chi. Molecular characterization of an anti-epilepsy peptide from the scorpio Buthus martensi Karsch. Eur. J. Biochem. 268, 2480, 2001.
  20. Xiang-Dong Fang, Chun Chen, Qi Wang, Jian-Xin Gu, Cheng-Wu Chi. The Interaction of the Calcium and Integrin Binding Protein (CIBP) with the Coagulation Factor VIII. Thromb. Res. 102, 177, 2001.
  21. Hao Fei, Yan LI, Li-Xiu Wang, Ming-Juan Luo, Min-Hua Ling, Cheng-Wu Chi. Purified nonhistone protein from porcine kidney acts as a suicide substrate inhibitor on furin-like enzyme. Acta Pharmacol. Sin. 21, 265, 2000.
  22. Li Dai, Jing-jiang Wu, Yi-hua Gu, Zheng-dao Lan, Min-hua Ling and Cheng-wu Chi. Genomic organization of three novel toxins from the scorpion Buthus martinsi Karsch that are active on potassium channels. Biochem. J. 346, 805, 2000.
  23. Jing-jiang Wu, Li Dai, Zheng-dao Lan and Cheng-wu Chi. The Gene Cloning and Sequencing of Bm-12, a Chlorotoxin-like Peptide from the Scorpion Buthus martinsi Karsch. Toxicon 38, 661, 2000.
  24. Feng Shao, Yu-mei Xiong, Rong-huan Zhu, Min-hua Ling, Cheng-wu Chi & Da-cheng Wang. Expression and Purification of the BmK M1 Neurotoxin from the Buthus martinsi Karsch. Protein Expression and Purification 17, 358, 1999.
  25. Yan-shi Zhu, Qi-chen Huang and Cheng-wu Chi. Crystal structure of mung bean inhibitor lysine active fragment complex with bovine beta-trypsin at 1.8A resolution. J Biomol Struct Dyn. 1999 Jun;16(6):1219-24.
  26. Chun Chen, Xiao-dong Fang, Jiang Zhu, Xiang-fu Wu, Zu-chuan Zhang, Zheng-yi Wang and Cheng-wu Chi. The Gene Expression of Coagulation Factor VIII in Mammalian Cell Lines. Thrombosis Res. 95, 105, 1999.
  27. Jing-jiang Wu, Li Dai, Zheng-dao Lan and Cheng-wu Chi. Genomic Organization of Three Neurotoxins Active on Small Conductance Ca++-activated Potassium Channels from the Scorpion Buthus martinsi Karsch. FEBS Letters 452, 360, 1999.
  28. Yu-mei Xiong, Zheng-dao Lan, Miao Wang. Bo Liu, Xin-qi Liu, Hao Fei, Lai-gen Xu, Qi-Chang Xia, Chung-guang Wang, Da-cheng Wang and Cheng-wu Chi. Molecular Characterization of a New Excitatory Insect Neurotoxin with an Analgesic Effect on Mice from the Scorpion Buthus martinsi Karsch. Toxicon 37, 1165, 1999.
  29. Zheng-dao Lan, Li Dai, Xiao-liang Zhuo, Ke Xu, Jun-Cai Feng and Cheng-wu Chi. Gene Cloning and Sequencing of BmK AS and BmK As-1, Two Novel Neurotoxins from the Scorpion Buthus martinsi Karsch. Toxicon. 37, 815, 1999.
  30. Yu-mei Xiong, Min-hua Ling, Zheng-dao Lan, Da-cheng Wang and Cheng-wu Chi .The cDNA Sequence of an Excitatory Insect Selective Neurotoxin from the Scorpion Buthus martinsi Karsch. Toxicon 37, 335, 1999.
  31. Yu-mei Xiong, Min-hua Ling, Da-cheng Wang and Cheng-wu Chi. The cDNA and Genomic DNA Sequences of a Mammalian Neurotoxin from the Scorpion Buthus martensii Karsch. Toxicon 35, 1025, 1997.
  32. Ming-juan Luo, Yu-mei Xiong, Miao Wang, Da-cheng Wang and Cheng-wu Chi. Purification and Sequence Determination of a New Neutral Mammalian Neurotoxin from Scorpion Buthus martensii Karsch. Toxicon 35, 723, 1997.
  33. Ming-juan Luo, Wu-yuan Lu and Cheng-wu Chi. Clarification of an Uncertain Intron within the cDNA Sequences of Arrowhead Proteinase Inhibitor A and B. J. Biochem. (Tokyo) 121, 991, 1997.
  34. Zhi-wei Xie, Ming-juan Luo, Wen-feng Xu and Cheng-wu Chi. Two Reactive Site Location and Structure-Function Study of the Arrowhead Protease Inhibitors,A and B, Using Mutagenesis. Biochemistry (US) 36, 5846, 1997.

所长信箱 | 联系我们 | 机票预订 | 相关链接 | 上海生命科学研究院 | 中国科学院

Copyright 2017-2020 中国科学院生物化学与细胞生物学研究所 版权所有

沪ICP备05033115号